DNA damage of germ cell of rat induced by nitrotoluene chemicals  

作　　者：YANGLi XUJing-bo ZHENLin SUNZhi-wei SHILong JINMing-hua LIUXiao-mei 

机构地区：[1]InstituteofGeographicalSciencesandNaturalResourcesResearch,ChineseAcademyofSciences,Beijing100101,China／／GraduateSchooloftheChineseAcademyofSciences,Beijing100039,China [2]DepartmentofEnvironmentalScience,NortheastNormalUniversity,Changchun130024,China [3]InstituteofGeographicalSciencesandNaturalResourcesResearch,ChineseAcademyofSciences,Beijing100101,China [4]SchoolofPublicHealth,JilinUniversity,Changchun130021,China

出　　处：《Journal of Environmental Sciences》2005年第1期84-90,共7页环境科学学报（英文版）

基　　金：TheKeyProjectoftheMinistryofEducationofChina(No.01063)andtheNationalNaturalScienceFoundationofChina(No.30230090;30370258)

摘　　要：dinitrotoluene(2,4 DNT), 2,6 dinitrotoluene(2,6 DNT) and 4 nitrotoluene(4 NT) are typical pollutants in the Songhua River of Northeast China. Sertoli/germ cell cocultures and single cell gel electrophoresis(SCGE) are applied to investigate whether they have genotoxicity on DNA damage of germ cell of Kunming male rat. The results showed that all three nitrotoluene compounds tested could induce DNA single strand breaks of the germ cell. A significant relationship is found between logarithm dose and the degree of DNA damage, which implies that 2,4 DNT, 2,6 DNT and 4 NT have genotoxicity and can induce the germ cell DNA strand to break in vitro.dinitrotoluene(2,4 DNT), 2,6 dinitrotoluene(2,6 DNT) and 4 nitrotoluene(4 NT) are typical pollutants in the Songhua River of Northeast China. Sertoli/germ cell cocultures and single cell gel electrophoresis(SCGE) are applied to investigate whether they have genotoxicity on DNA damage of germ cell of Kunming male rat. The results showed that all three nitrotoluene compounds tested could induce DNA single strand breaks of the germ cell. A significant relationship is found between logarithm dose and the degree of DNA damage, which implies that 2,4 DNT, 2,6 DNT and 4 NT have genotoxicity and can induce the germ cell DNA strand to break in vitro.

关 键 词：nitrotoluenes sertoli/germ cell cocultures SCGE GENOTOXICITY 

分 类 号：X503.22[环境科学与工程—环境工程] X171.5