木聚糖酶的定向制备及其在制浆造纸工业中的应用  被引量:3

Xylanase Selectively Preparation and Application in Pulp and Paper Industry

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作  者:勇强[1] 尤纪雪[1] 顾祺萍[1] 刘超纲[1] 余世袁[1] 

机构地区:[1]南京林业大学化学工程学院,江苏南京210037

出  处:《中国造纸学报》2004年第2期44-47,共4页Transactions of China Pulp and Paper

基  金:国家自然科学基金资助项目 (30 1 0 0 1 4 2 )

摘  要:里氏木霉以 7g/L木聚糖为碳源制备低纤维素酶活木聚糖酶 ,木聚糖酶活、CMC酶活和木聚糖酶与CMC酶的酶活比在培养 2天时分别为 95 1IU/mL、0 2 58IU/mL和 368 6 ;延长产酶时间到第 4天 ,酶活比提高到 1 4 0 1 1。当木聚糖酶用于漂白预处理 ,且其用量为 5IU/g时 ,可使漂白纸浆白度由 76 5 %SBD提高至 82 0 %SBD ,而卡伯值由 4 31降至 2 1 3;当木聚糖酶用于废纸脱墨且其用量为 3IU/g时 ,与对照浆相比 ,脱墨浆白度提高了 2 8%SBD、尘埃度下降了 71 5 % ,同时在酶用量为 1~ 9IU/g范围内 ,脱墨浆的裂断长、耐破指数和撕裂指数均比原浆有所提高。Low cellulase containing xylanase could be prepared by Trichoderma reesei. The activities of xylanase, CMCase and xylanase/CMCase were 95.1 IU/mL, 0.258 IU/mL and 368.6 respectively for 2-day cultivation, the xylanase/CMCase increased to 1401.1 while the fermentation time increased to 4 days. When the low cellulase containing xylanase was used for biobleaching and the pulp was pretreated with the dosage of 5 IU/g pulp before bleaching, the brightness of the pulp increased from 76.5%SBD to 82.0%SBD and the kappa value decreased from 4.31 to 2.13 compared with the conventional bleaching. The result of xylanase deinking, which the optimal dosage was 3 IU/g pulp, was that the brightness of deinked pulp increased 2.8%SBD and the dirt count decreased 71.5 % compared with that of the control pulp. Meanwhile, the breaking length, burst index and tear index were improved after bio-deinking when 1~9 IU/g pulp xylanase was used.

关 键 词:木聚糖酶 脱墨浆 白度 CMC酶活 制浆造纸工业 裂断长 尘埃度 用量 碳源 里氏木霉 

分 类 号:TS745[轻工技术与工程—制浆造纸工程] S816[农业科学—饲料科学]

 

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