氧化砷和顺铂对喉鳞癌细胞Hep-2的作用及其机制  

Study the effect of arsenous oxide and cisplatin on laryngeal squamous cell carcinoma Hep-2 strain and the effective mechanism

作　　者：肖辉[1] 金德钧[1] 王超[1] 徐秀玉[1] 杨静波[2] 李呼伦[3] 

机构地区：[1]哈尔滨医科大学第二临床医学院耳鼻咽喉科,黑龙江哈尔滨150086 [2]哈尔滨市第三医院,黑龙江哈尔滨150066 [3]哈尔滨医科大学神经生物学教研室,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2004年第6期540-542,共3页Journal of Harbin Medical University

摘　　要：目的　探讨氧化砷 (arsenousoxide ,As2 O3)和顺铂 (cisplatin ,DDP)对喉鳞癌细胞 (Hep 2 )的作用及其机制。方法　用Hep 2细胞株进行传代培养 ,观察As2 O3和DDP对不同培养时间细胞的生长抑制情况 ;MTT法检测不同浓度的As2 O3和DDP对Hep 2细胞存活率的影响 ,并记录细胞形态的变化 ;电镜和 3’ 原位末端标记法 (TUNEL)检测Hep 2细胞凋亡的发生和形态学改变。结果　①随着药物对Hep 2细胞作用时间的延长 ,细胞生存率明显降低 ,存在时间依赖性。②MTT检测显示 ,As2 O3和DDP对细胞增殖的抑制效应具有剂量依赖性。③光镜观察发现 ,药物作用组细胞出现病变。④电镜和TUNEL染色证实 ,As2 O3和DDP对Hep 2细胞的抑制作用是以促进细胞凋亡为主。结论　As2 O3和DDP可能通过促进细胞凋亡发挥抗肿瘤的作用。Objective To study the effect and the mechanism of arsenous oxide (As 2O 3) and cisplatin (DDP) on laryngeal squamous cell carcinoma Hep-2 strain.Methods The laryngeal squamous cell carcinoma Hep-2 strain was chosen in this experiment.The inhibitory effects of As 2O 3 and DDP on Hep-2 strain were assayed with MTT test and recorded the changes of cultured cells.The form of Hep-2 cells was observed by electron microscope and the apoptosis was detected by TUNEL test.Results ①After the administration of As 2O 3 and DDP,the survival rate of Hep-2 cells decreased significantly with time-dependent.②As 2O 3 and DDP inhibit the proliferation of Hep-2 cells with dose-dependent.③The cells of As 2O 3 and DDP group had pathological changes.④The fact that apoptosis was a major way of Hep-2 cell death after drug treatment were confirmed by TUNEL test and electron microscope.Conclusion As 2O 3 and DDP may suppress carcinoma by accelerating apoptosis.

关 键 词：氧化砷 顺铂 喉鳞癌细胞 凋亡 

分 类 号：R739.65[医药卫生—肿瘤]