三氧化二砷经肝动脉栓塞化疗对兔肝移植瘤的作用  

Effects of Arsenic Trioxide by Transcatheter Arterial Chemoembolization on Rabbits with Hepatic Vx-2 Carcinoma

作　　者：郑明友[1] 于晓东[2] 汪涛[1] 谢成兵[2] 黎光荣[1] 王吉生[2] 何世举[1] 

机构地区：[1]重庆市第三人民医院肝胆外科,400014 [2]重庆市第三人民医院病理科,400014

出　　处：《肿瘤防治研究》2004年第12期751-753,共3页Cancer Research on Prevention and Treatment

摘　　要：目的　探讨As2 O3 经肝动脉栓塞化疗对肝移植瘤的作用。方法　采用实验组 1(生理盐水 )、实验组 2 (碘化油 )、实验组 3(As2 O3 +碘化油 )经肝动脉注射治疗肝移植瘤一次 ,实验组 4 (As2 O3 )经肝动脉插管灌注药物 (连续 7天 )。观察肿瘤平均瘤重、平均瘤重抑制率、肿瘤细胞形态学变化 ,检测bcl 2 /bax基因表达和VEGF表达。结果　实验组平均瘤重分别为 16 .0 8± 2 .6 9g、3.4 4± 0 .84 g、1.5 2± 0 .2 6 g和4 .87± 0 .73g ,平均瘤重抑制率分别为 78.6 1%、90 .5 5 %和 6 9.71% ,实验组 3移植瘤瘤重低于其他实验组 (P <0 .0 5 )。实验组 3、4移植瘤组织bax基因表达显著上调、bcl 2基因表达显著下调 (P <0 .0 5 )、VEGF表达下调 (P <0 .0 5 ) ;电镜观察实验组 3、4均有典型的瘤细胞凋亡改变。结论　As2 O3 经肝动脉栓塞化疗对兔Vx 2肝移植瘤有显著抑瘤作用 ,碘化油能增强As2 O3 对肝移植瘤的抑制作用 ,两药具有协同作用。Objective To evaluate the anticancer effect of arsenic trioxide(As 2O 3) by transcatheter arterial chemoembolization (TACE) on rabbits with hepatic Vx-2 carcinoma. Methods Forty New Zealand rabbits with hepatic Vx-2 carcinoma were managed with normal saline (experimental group 1), lipiodol and normal saline (experimental group 2), As 2O 3 and lipiodol (experimental group 3) by hepatic artery infusion respectively, whereas experimental group 4 was treated with As 2O 3 by TAI for 7 days consecutively. Four weeks later, the tumor weighty and its growth inhibitory rate was assessed respectively. The morphological changes of the tumor were observed under transmission electron microscope. bax/bcl-2 and VEGF expression were examined by immunohistochemistry. Results Average weighty of the tumor were 16.08± 2.69 g, 3.44± 0.84 g, 1.52± 0.26 g and 4.87± 0.73 g, and it's growth inhibitory rate in experimental group 2,3,4 were 78.61%, 90.55% and 69.71% respectively. The growth of tumor were obviously inhibited in experimental group 3 compared with the experimental group 1,2,4 (P< 0.05). The upregulated bax expression and downregulated bcl-2 expression were detected in experimental group3,4 by immunohistochemistry, comparison with experimental group 1,2 (all P< 0.05). Significantly decreased VEGF expression was also observed in experimental groups (P< 0.05). Some tumor cells of experimental group3,4 were showed the typically morphological characteristics of apoptosis under transmission electron microscope. Conclusion As 2O 3 by TACE can effectively inhibit hepatic Vx-2 carcinoma cells on rabbit's. Lipiodol strongly potentiates As 2O 3 inhibited the growth of hepatic Vx-2 carcinoma cells on rabbit,s, and the two drugs can produce a significant synergic effect.

关 键 词：三氧化二砷 肝动脉栓塞化疗 肿瘤移植 肝肿瘤 兔 

分 类 号：R735.8[医药卫生—肿瘤] R73-31[医药卫生—临床医学]