红光下氨苄青霉素对菊花水培插枝生根和生理作用  

Effect of Ampicillin and Red Light on the Chrysanthemum's Root Formation and Physiological Active

作　　者：刘亚丽[1] 孙富丛[1] 刘萍[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《生物技术》2004年第6期59-62,共4页Biotechnology

基　　金：新乡市科技发展计划项目 (0 4N0 1 5)资助

摘　　要：用氨苄青霉素处理的菊花茎段 ,分别放在红光和自然光下的生根和生理作用 ,目的是探索红光与氨苄青霉素对菊花的生根和生理作用是否有叠加作用。将菊花茎段在不同浓度 (10 0、2 0 0、30 0、4 0 0mg·L-1)的氨苄青霉素中浸泡 12h ,再分别在红光和自然光下进行水培 ,各用自来水作对照。结果表明 :红光下各处理比自然光下各处理茎段粗壮、生根早、根系发达、生根率高、根多且长。红光下相对电导率、MDA含量、IAA氧化酶活性均低 ,叶绿素含量高。故红光下 2 0 0mg·L-1氨苄青霉素浓度是促进茎段生根、控制相对电导率和MDA含量升高。This experiment uses chrysanthemum as material.Put the chrysanthemum stems which are conditioned by ampicillin into red light and natural light to test the root formation and physiological active.The purpose is to make sure if ampicillin and red light could make the root formation and physiological active of chrysanthemum stems better together.Put the stems of the chrysanthemum into different types of ampicillin solution whose concentration are 100mg·L^(-1),200mg·L^(-1),300mg·L^(-1) and 400mg·L^(-1) for 12 hours.Then water cultures them respectively in red light and in natural light and contrast with tap water.The result shows that the stems in red light have earlier root formation,higher root formation rate,more roots,better growth potential,more Chl in leaves and more amount of soluble sugar than the stems in natural light.What's more,relative conductivity,MDA content,IAA oxidase are lower in red light.So the kind of ampicillin which concentration is 200mg·L^(-1) can do the best to help the stems to take root,decrease relative electrical conductivity and the content of MDA and lower the activity of IAA oxidase in red light.

关 键 词：氨苄青霉素 插枝生根 红光 自然光 菊花 MDA 相对电导率 IAA氧化酶 叶绿素 

分 类 号：Q945.34[生物学—植物学]