猪Pit-1基因第三内含子序列的克隆测序  

Cloning and Sequencing of Pig Pit-1 Gene intron3 Sequence

作　　者：庞瑾[1] 郑友民[2] 李宏滨[1] 

机构地区：[1]中国农业科学院畜牧研究所,北京100094 [2]中国农业科学院农业质量标准与检测技术研究所,北京100081

出　　处：《生物技术通报》2004年第6期41-43,共3页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目 (编号 3 9970 5 42 );"九五"国家重点科技攻关项目--- 973项目 (编号TG2 0 0 0 0 1610 4)资助

摘　　要：根据不同物种间同一基因核苷酸序列的保守性及相似性的特点 ,在人和鼠的Pit 1基因第三外显子上设计上游引物 ,而将下游引物设计在猪Pit 1基因第四外显子上。利用聚合酶链式反应 (PCR)技术 ,扩增出猪Pit 1基因第三内含子序列 ,并经由酶切及序列同源性比对确认该序列即为猪Pit 1基因第三内含子序列。According to the character that the same nucleic acid sequence has conservatism and similarity in different species, designed the sense primer in exon3 sequence of human and mouse Pit-1 gene .Thus, designed the anti-sense primer in exon4 sequence of pig Pit-1 gene. Then PCR reaction was used to amplify the intron3 sequence of pig Pit-1 gene, and further identified this sequence by digesting with EcoRI and comparing the identity of this sequence with other species. This sequence will provide some of basal information to research the pig Pit-1 gene further.

关 键 词：基因 Pit-1 外显子 聚合酶链式反应 扩增 内含子 PCR) 克隆测序 物种 序列同源性 

分 类 号：S828[农业科学—畜牧学]