转化生长因子β_1对牛角膜内皮细胞DNA合成的影响  

Effects of transforming growth factor beta-1 on DNA synthesis of bovine endothelial cells

作　　者：严浩[1] 胡燕华[1] 黄琼[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,湖北武汉430022

出　　处：《眼视光学杂志》2004年第4期250-252,254,共4页Chinese Journal of Optometry & Ophthalmology

摘　　要：目的 :研究外源性转化生长因子 β1(transforminggrowthfactor,TGF β1)对牛角膜内皮细胞 (bovinecornealen dothelialcells,BCECs)增殖过程的影响。方法 :在体外培养的牛角膜内皮细胞经不同浓度 (0 .10、1.0 0、10 .0 0、10 0 .0 0 μg/L)TGF β1处理后 ,用流式细胞术测量分析细胞周期 ,用甲基噻唑基四唑 (methylthiazolyltetrazolium ,MTT)法检测TGF β1对角膜内皮细胞增殖功能以及DNA合成的影响。结果 :一定浓度 (0 .10、1.0 0、10 .0 0 μg/L)的TGF β1对角膜内皮细胞有明显的促增殖作用 ,并呈剂量依赖性。但在较高浓度 (10 0 .0 0 μg/L)时对内皮细胞表现出一定的抑制作用。结论 :一定浓度外源性的TGF β1与其受体结合后可以促进牛角膜内皮细胞DNA的合成 ,可以参与角膜内皮细胞的损伤修复再生过程。Objective:To study the effects of transforming growth factor beta 1(TGF β 1) on the proliferation of bovine corneal endothelial cells(BCECs).Methods:BCECs in culture were treated with different concentrations of TGF β 1(0.10、1.00、10.00、100.00 μg·L -1 ),2 days after treatment with TGF β 1. An MTT assay of 570 nm was used to examine cell proliferation and flow cytometry was used to analyze the cell cycle.Results:At concentrations of TGF β 1 from 0.1 μg·L -1 to 10.00 μg·L -1 ,cell proliferation was enhanced compared to the control group(P<0.01),and the growth of endothelial cells was enhanced by TGF β 1 in a dose dependent manner. But at higher concentrations(100.00 μg·L -1 ),TGF β 1 inhibited corneal endothelial cell proliferation.Conclusion:Exogenous TGF β 1 combined with its receptors can stimulate DNA synthesis of bovine corneal endothelial cells in vitro. Perhaps TGF β 1 contributes to the wound healing process in corneal endothelial cells. [

关 键 词：角膜内皮细胞 TGF-Β1 DNA合成 转化生长因子Β1 增殖 影响 受体结合 对角 结论 参与 

分 类 号：R77[医药卫生—眼科]