不同产地西洋参皂甙成分的HPLC分析  

HPLC ANALYSIS OF SAPONINS AMERICAN GINSENG PRODUCED IN DIFFERENT AREAS

作　　者：苏建[1] 李海舟[2] 孔令义[3] 杨崇仁[2] 

机构地区：[1]云南省药品检验所,昆明650011 [2]中国科学院昆明植物研究所,昆明650204 [3]中国药科大学,南京210009

出　　处：《天然产物研究与开发》2004年第6期561-564,共4页Natural Product Research and Development

摘　　要：以西洋参的主要皂甙成分人参皂甙Re和Rb1为标准对照品 ,建立西洋参药材的HPLC定量分析技术 ,并参考人参皂甙Rc,Rd及Rg2 的相对峰面积进行主成分分析。色谱条件为 :C18柱 (5 μm ,3.9× 15 0mm) ,乙腈 :水流动相 ,二元梯度洗脱 ,检测波长 2 0 3nm。结果表明 ,就皂甙成分的组成与含量而言通过人参皂甙Re和Rb1的含量测定和皂甙的主成分分析 ,不同产地的西洋参药材皂甙成分存在一定的差别。吉林省靖宇县产的西洋参与进口品最为接近。To control the quality of American Ginseng,HPLC was carried out with C18 column(5 μm,3.9×150 mm) using acetonitrile and water as mobile phase, and detected at 203 nm.Ginsenoside Re and Rb 1 were used as the standard for quantitative analysis.The relative contents of Ginsenoside Rc, Rd and Rg 2 were used for principle components analysis (PCA).Distinct differences were found among American Ginseng produced in different places through quantitative analysis and PCA.American Ginseng produced in Jingyu, Jilin province is the mostly close one to those imported in view of glycosides and their contents.

关 键 词：不同产地 西洋参 RB1 人参皂甙RE 对照品 色谱条件 HPLC C18柱 药材 成分 

分 类 号：Q343.1[生物学—遗传学] R282[医药卫生—中药学]