杀菌剂抗性分子检测技术的研究进展  

The Progress of DNA-based Approaches for Detection of Fungicide Resistance

作　　者：李红霞[1] 王建新[1] 周明国[1] 

机构地区：[1]南京农业大学植物保护学院,江苏南京210095

出　　处：《农药学学报》2004年第4期1-6,共6页Chinese Journal of Pesticide Science

基　　金：国家高技术发展研究计划(863计划)(2002AA244041;2004AA249040);国家自然科学基金(30200181);南京农业大学青年科技创新基金(KJ04004).

摘　　要：靶标病菌基因突变是许多内吸性杀菌剂出现抗性的根本原因,检测与抗药性相关的靶标病菌基因突变对阐明抗药性发生的分子生物学机制及进行早期诊断具有重要意义。目前已成功用于检测靶标病菌抗药性菌株的分子检测技术有6种:等位基因特异性扩增、限制性片段长度多态性、等位基因特异性寡核苷酸杂交、单链构象多态性、实时定量PCR、变性高效液相色谱分析。这些技术能够快速、灵敏地检测田间早期出现的抗药性或抗药性种群的发展动态,在病害的可持续管理系统中科学使用杀菌剂方面发挥着重要作用。Gene mutation is the fundamental reason why many plant phytopathogenic fungi show the resistance to lots of systemic fungicides. It is important to detect relative gene mutation to (reveal) molecular mechanism of fungicide resistance and earlier diagnosis. Six molecular (techniques) have been successfully employed to detect resistant isolates of target fungus, such as Allele-Specific　Amplification(ASA), Restriction Fragment Length Polymorphism(PCR-RFLP), (Allele-Specific) Oligonucleotide Hybridization(ASOH), Single-Strand Conformation Polymorphism(SSCP), Quantitative Real-time PCR, Denaturing High-Performance Liquid Chromatography ((DHPLC).) These modern techniques can detect earlier fungicide resistance or development of (resistant) sub-population in field timely, quickly and accurately. The increasing use of diagnostic tools to detect fungicide resistance plays an important part in improving the rational use of (fungicides) for sustainable disease management systems.

关 键 词：靶标病菌 基因突变 杀菌剂抗性 分子检测 

分 类 号：S481.4[农业科学—农药学]