灵芝多糖肽对小鼠腹腔巨噬细胞一氧化氮产生的影响  

The effects of ganoderma lucidum polysaccharides peptide (GLPP) on the nitric oxide production in mice peritoneal macrophages

作　　者：游育红[1] 林志彬[1] 

机构地区：[1]北京大学医学部药理系,北京100083

出　　处：《中国药理学通报》2004年第12期1398-1401,共4页Chinese Pharmacological Bulletin

基　　金：上海绿谷 (集团 )有限公司科研基金资助项目

摘　　要：目的　研究灵芝多糖肽 (GLPP)对小鼠腹腔巨噬细胞一氧化氮产生的影响并探讨其作用机制。方法　以Griess法 ,观察GLPP对LPS诱导小鼠腹腔巨噬细胞一氧化氮(NO)产生的影响 ;以免疫组化法检测诱导型一氧化氮合成酶 (iNOS)的表达 ,观察GLPP对iNOS的影响。结果　GLPP(2 5～ 2 0 0mg·kg-1)灌胃给药 5d或体外给药 (3 12 5～ 2 0 0mg·L-1)均可促进巨噬细胞NO释放 ,但对LPS刺激NO的释放影响不大 ;GLPP(10 0mg·kg-1)灌胃给药 5d或体外给药 (10mg·L-1)均可使巨噬细胞iNOS含量增加。结论　GLPP可增加小鼠腹腔巨噬细胞NO产生 ,其机制可能与其促进巨噬细胞iNOS合成有关。Aim To study the effects of Ganoderma polysaccharide peptide (GLPP) on the production of nitric oxide (NO) in mice peritoneal macrophages induced by LPS and its mechanism. Methods The effects of GLPP on the NO releasing from mice peritoneal macrophages induced by LPS were detected by using Griess reagent and the expression of iNOS by GLPP in mice peritoneal macrophages was detected by immunohistochemical method.Result The production of NO was increased by GLPP.(25～200 mg·kg -1) ig for five days or by GLPP(3.125～200 mg·L -1) in vitro but the effects of LPS on the production of NO was not influenced significantly. The expression of iNOS was increased by GLPP(25～200 mg·kg -1) ig for five days or GLPP(3.125～200 mg·L -1) in vitro.Conclusion GPP in vivo or in vitro could increase the production of NO in mice peritoneal macrophages. It might take effects by enhancing the synthesis of iNOS.

关 键 词：灵芝多糖肽 一氧化氮 腹腔巨噬细胞 免疫组化 一氧化氮合成酶 

分 类 号：R-332[医药卫生] R282.71