结肠癌患者癌组织中CD44v6和Ⅳ型胶原的表达及血清中HA和Ⅳ型胶原的定量检测  

Expression of CD44v6 and type Ⅳ collagen in tissues, concentrations of serum hyaluronic acid and type Ⅳ collagen in patients with colorectal carcinoma

作　　者：徐钊[1] 张霞[1] 房殿春[1] 晋华源[1] 罗元辉[1] 阎晓初[1] 王东旭[1] 

机构地区：[1]中国人民解放军第三军医大学西南医院全军消化专科中心,重庆市400038

出　　处：《世界华人消化杂志》2004年第10期2312-2315,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨结肠癌组织中CD44v6和Ⅳ型胶原的表达以及血清中透明质酸(HA)和Ⅳ型胶原的浓度在结肠癌预后判断中的作用.方法:采用免疫组织化学技术,检测60例结肠癌组织以及10名正常组织中CD44v6和Ⅳ型胶原的表达,同时采用放射免疫技术检测上述60例结肠癌及10名正常人血清中透明质酸(HA)和Ⅳ型胶原的含量.结果:结肠癌组织中CD44v6阳性率为75%,明显高于癌旁对照及正常结肠组织,有淋巴结转移组的结肠癌组织中CD44v6的表达明显高于无转移组(70.9%vs33.3%,P<0.01),DukeA和DukeB期明显低于DukeC和DukeD期(30.3%,36.4%vs62.5%,66.7%,P<0.01):而Ⅳ型胶原在结肠癌组织中均有不同程度的减少,其中高分化组结肠癌组织中明显高于中、低分化组(61.1%VS29.0%,18.1%,P<0.01),有淋巴结转移组明显低于无淋巴结转移组(20.8%vs58.3%,P<0.01),DukesA和DukeB期明显高DukeC和DukeD期(70.0%,54.5%vs31.3%,16.6%.P<0.01),结肠癌患者血清中HA和Ⅳ型胶原含量明显高于正常对照组(200±160μg/L,125±42μg/Lvs61±14μg/L,60±15μg/L,P<0.01),且与有无淋巴结转移和Dukes分期相关,随着组织中CD44v6和Ⅳ型胶原表达逐渐增加,血清中HA亦逐渐增加,而Ⅳ型胶原则逐渐减少.结论:组织中CD44v6和Ⅳ型胶原的表达及血清中HA和Ⅳ型胶原的含量对判断结肠癌患者的预后有重要意义.AIM: To explore the role of CD44v6, type Ⅳ collagen expression in tissues and concentration of serum hyaluronic acid (HA) and type Ⅳ collagen in predication of prognosis of patients with colorectal carcinoma. METHODS: Expression of CD44v6 and type Ⅳ collagen in the carcinoma tissues (n =60) and normal tissues (n =10) were detected by immunohistochemistry. Meanwhile, concentrations of HA and type Ⅳ collagen in serum were tested with radioimmunology. RESULTS: Positive rate of CD44v6 in colorectal tissues was 75%, which was significantly higher than that in adjacent or normal tissues. Expression of CD44v6 in patients with lymph node metastasis was significantly higher than that without metastasis; expression of CD44v6 in Duke C or Duke D stage was significantly higher than that in Duke A or Duke B stage (62.5%, 66.7% vs 30.3%, 36.4% respectively, P <0.01). Expression of type IV collagen in poor or moderate differentiation tissues was significantly lower than that in well-differentiation ones (29.0%, 18.1% vs61.1%;P<0.01). Expression of type Ⅳ collagen in Duke C, Duke D stage or in tissues with lymph node metastasis was significantly lower than that in Duke A, Duke B stage or that in tissues without lymph node metastasis (31.1%, 16.6%, 20.8% vs70.0%; 54.5%, 58.3%, respectively; P <0.01). Concentrations of serum HA and type IV collagen in patients with colorectal cancer were significantly higher than those in normal group (200 ± 160,125 ± 42 μg/L vs61±14, 60 ±15 μg/L,P<0.01) and they related to lymph node metastasis and Dukes staging. With the increased expression of CD44v6 and type Ⅳ collagen in tissues of patients with colorectal cancer, the concentration of serum HA increased, whereas the concentration of serum type Ⅳ collagen decreased. CONCLUSION: Expression of CD44v6 and type Ⅳ collagen in tissues and concentrations of serum HA and type Ⅳ collagen are useful parameters in predication of prognosis of patients with colorectal carcinoma.

关 键 词：结肠癌 癌组织 CD44V6 Ⅳ型胶原 表达 血清 HA Ⅳ型胶原 定量检测 

分 类 号：R735.3[医药卫生—肿瘤]