睾酮对大鼠再生肝细胞核仁组织区影响的初步研究  

Effect of Testosterone on Nucleolar Organizer Regions in Regenerating Hepatocytes in Rat: a Preliminary Study

作　　者：李恩中[1] 宁黔冀[1] 李超堃[1] 

机构地区：[1]河南师范大学生命科学院,新乡453002

出　　处：《动物学杂志》2004年第6期82-86,共5页Chinese Journal of Zoology

基　　金：河南省自然科学基金(No.20011800009);河南省高校青年骨干教师资助计划(200195)

摘　　要：以银染核仁组织区(argyrophilnucleolarorganitingregions,AgNORs)阳性颗粒数为指标,结合放射免疫技术,研究了大鼠部分肝切除(partialhepatectomy,PH)后,睾酮对余留肝细胞核仁组织区的影响。结果显示,pH后0～24h,各种方式(假手术、皮下注射芝麻油或睾酮)处理组,AgNORs数都下降,随后持续升高。在PH后48h和72h,低剂量(05mgkg体重)睾酮对AgNORs数影响最显著,都明显高于其它两个睾酮处理组;中等剂量(25mgkg体重)和高剂量睾酮(5mgkg体重)处理对AgNORs数的影响与芝麻油处理组相比都无显著差异,但均高于假手术组;假手术后72h的AgNORs恢复到术前水平。血清睾酮含量测定显示,芝麻油处理组PH后0～48h睾酮持续下降,48h后不再下降,48h和72h时的睾酮浓度显著低于假手术组;低剂量处理,血清睾酮浓度在PH后0～24h下降,然后持续上升,在48h、72h达到假手术组的2～3倍;中等剂量和高剂量处理组,血清睾酮在PH后0～72h持续升高,是假手术组的6～7倍。以上结果初步表明,睾酮对PH后肝细胞中AgNORs的影响有两种情况(1)在血清中的浓度是生理水平的2～3倍时,起促进作用;(2)6～7倍于生理水平时,基本无作用。The effect of testosterone on nucleolar organizer regions in rat regenerating hepatocytes after partial hepatectomy(PH) was studied by using Argyrophil nucleolar organizing regions(AgNORs) as the end point.The results indicated that the number of AgNORs decreased in both sham hepatectomized rats and hepatectomized rats treated with testosterone or sesame oil replacement during 0-24?h after operation. After 24?h, the number of AgNORs increased constantly. Treatment with exogenous testosterone at a low dose (0 5?mg/kg body weight) had the most remarkable influence on AgNORs number in all groups, and the AgNORs number in low dose testosterone treated group was significantly higher than that of two testosterone treated groups at 48?h and 72?h post partial hepatectomy. No significant difference was observed when compared middle or high dose (2 5 or 5 ?mg/kg body weight) testosterone treated group to sesame oil treated group, while the number of AgNORs in all three groups was higher than that of sham operated rats. The AgNORs number in sham operated rats at 72?h declined to that at 0?h. Measurement of serum testosterone indicated that testosterone in sesame oil treated rats declined constantly during 0-48?h following PH (from 2 64±1 08 at 0?h to 1 02±1 05 at 48?h), and the concentration was lower than that in sham operated rats at 48?h and 72?h after hepatectomy. In low dose testosteronetreated rats, testosterone concentration decreased during 0-24?h after PH, then increased constantly and reached 2-3 folds of that of sham operated animals at 48?h and 72?h. These results suggest that there are two different effects of testosterone on transcriptional activity of regenerating hepatocytes in rat following PH. When the serum testosterone concentration is 2-3 times of the physiological level, it facilitates transcriptional activity; while when serum testosterone concentration reaches 6-7 times of the physiological level, it has no evident effect on transcriptional activity

关 键 词：大鼠 部分肝切除 睾酮 核仁组织区 

分 类 号：Q459[生物学—生理学] R335.5[医药卫生—人体生理学]