Chl H/RS细胞与背景细胞蛋白表达的相关性  

Study on protein expression of H/RS cell and it's background lymphocytes in classical Hodgkin's lymphoma

作　　者：吴自勍[1] 赵彤[2] 贺海容 朱梅刚[2] 

机构地区：[1]南方医科大学分校病理学教研室,广东省广州市510315 [2]南方医科大学病理学教研室,广东省广州市510515 [3]广州市海珠区二人民医院,广东省广州市510000

出　　处：《第一军医大学分校学报》2004年第2期91-94,共4页Journal of Branch Campus of the First Military Medical University

基　　金：国家自然科学基金 (39970 6 92 );广东省自然科学基金 (980 2 17)资助

摘　　要：目的 :探讨cHL肿瘤性H/RS细胞与反应性背景淋巴细胞蛋白表达的相关性。方法 :采用免疫组化SABC法对 32例cHL进行CD2 0、CD30、CD15、CD4 5RO和LMPI蛋白表达的检测。结果 :(1) 32例cHL中LrHL11例 ,NSHL2例 ,MCHL14例 ,LDHL5例 ;(2 ) 30 / 32 (93.8% )的H/RS细胞与 14 / 32 (4 3.8% )的北景淋巴细胞表达CD30 ,2 5 / 32J(78.1% )的H/RS细胞与 7/ 32(2 1.9% )的背景淋巴细胞表达CD15 6 / 32 (18.8% )的H/RS细胞与 8/ 32 (2 5 .0 % )的背景淋巴细胞优势表达CD2 0 ,7/ 32 (2 1.9% )的H/RS细胞与 18/ 32 (5 6 .3% )的背景淋巴细胞优势表达CD4 5RO ;(3) 2 0 / 32 (6 2 .5 % )的H/RS细胞表达LMP1蛋白。结论 :H/RS及其背景细胞的在蛋白表达水平上具有一定的相关性 ,检测CD2 0、CD30、CD15、CD4Objective:To investigate the relation between H/RS cell of Hodgkin's lymphom and it's background cells in protein expression.Methods:The expressions of CD15,CD30,CD20,CD45RO and LMP1 were detected in 32 cHL specimens by immunohistochemical techniques.Results:The results showed that(1)11 cases were LrHL,2 cases NSHL,14 cases MCHL,5 cases LDHL;(2)30/32(93.8%) of H/RS cells and 14/32(43.8%) of background lymphocytes were CD30 positive, 25/32(78.1%) of H/RS cells and 7/32(21.9%) of background B lymphocytes were CD15 positive, 6/32(18.8%) of H/RS cells and 8/32(25.0%) of background B lymphocyte predominance were CD20 positive, and 7/32(21.9%) of H/RS cells and 18/32(56.3%) of background T lymphocyte predominance were CD45RO positive; (3)20/32(62.5%) of H/RS cells were LMP1 positive.Conclusion:There are relations between Hodgkin's lymphoma and it's background cells in protein expression. The diagnosis and classification can be basicly verified by all these expressed markers of Hodgkin's lymphoma.

关 键 词：H/RS细胞 蛋白表达 淋巴细胞 CD20 CD30 HL CD15 背景 优势 结论 

分 类 号：R733[医药卫生—肿瘤] R730.21[医药卫生—临床医学]