红毛五加粗多糖脱蛋白工艺比较  

Comparison between Methods for Removal of Protein from Acanthopanax giraldii Harms Polysaccharrides.

作　　者：郭辉[1] 李善玲[1] 张红旭[1] 曲枫[1] 吕冬梅[2] 

机构地区：[1]江苏省徐州市第三人民医院,江苏徐州221005 [2]徐州医学院附属医院,江苏徐州221005

出　　处：《现代中药研究与实践》2004年第6期53-55,共3页Research and Practice on Chinese Medicines

基　　金：江苏省徐州市社会科技计划项目 ;编号 :X2 0 0 10 5 2

摘　　要：目的　研究红毛五加粗多糖脱蛋白工艺。方法　 1用 Sevage法脱蛋白 ;2用三氯乙酸法脱蛋白 ;3用苯酚 -硫酸法测定红毛五加多糖含量。结果　 1用 Sevage法脱蛋白后红毛五加粗品多糖中多糖含量为80 .6 % ;2用三氯乙酸法脱蛋白后红毛五加粗品多糖中多糖含量为 6 8.6 % ;。结论　用 Sevage法脱蛋白效率较高 ,可用于红毛五加多糖分离。Objective To compare methods for removing protein from Acanthopanax giraldii Harms Polysaccharrides. Method ①Protein was removed by using Sevage method;②Protein was removed by using Thicloroacetic acid;③The content of Acanthopanax giraldii Harms Polysaccharrides was determined by C 6H 6O- H 2SO 4. Result ①The content of Acanthopanax giraldii Harms Polysaccharrides was 80.6% by using Sevage method;②The content of Acanthopanax giraldii Harms Polysaccharrides was 68.6% after being removed by using Thicloroacetic acid. Conclusion Sevage method is more efficient.

关 键 词：蛋白 红毛五加多糖 粗多糖 多糖含量 三氯乙酸 苯酚-硫酸法 工艺比较 糖分 分离 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]