氯化饮水有机提取物对HepG2细胞的脂质过氧化作用  

EFFECT OF CHLORINATED DRINKING WATER EXTRACTS ON LIPID PEROXIDATION IN HEPG2 CELLS

作　　者：程晓莉[1] 程英惠 刘慧[1] 鲁文清[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院,武汉430030 [2]湖北省孝感市孝南区妇幼保健院,孝感432100

出　　处：《水生生物学报》2005年第1期38-42,共5页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金资助项目 (No 3 0 170 794)

摘　　要：东湖、汉江、长江是武汉市主要水源 ,本文研究了该三种水源的氯化饮水有机提取物对人肝肿瘤细胞HepG2的脂质过氧化作用 (LPO) ,探讨氯化饮水有机提取物引起细胞损伤的机制。以HepG2细胞作为靶细胞 ,采用体外培养细胞染毒方法 ,测定染毒细胞的丙二醛 (MDA)和还原型谷胱甘肽 (GSH)含量。氯化饮水有机提取物染毒浓度相当于 0 16 7、1 6 7、16 7、16 7mL水样 /mL培养液 ,DMSO(7μL/mL)为溶剂对照 ,H2 O2 (10 0 μmol/L)为阳性对照。与溶剂对照相比 ,氯化饮水有机提取物可使HepG2细胞脂质过氧化主要终产物MDA含量明显增加 ,抗氧化主要物质GSH明显下降 ,两者变化呈负相关。东湖氯化饮水有机提取物引起的MDA增加和GSH下降的作用较汉江明显 ,长江氯化饮水有机提取物对HepG2细胞的MDA则无明显影响。结果表明氯化饮水有机提取物对体外培养的HepG2细胞具有明显的脂质过氧化作用。比较而言 ,三种水源中东湖的氯化饮水有机提取物脂质过氧化作用较强。Dong(D)lake,Han(H)river and Yangtze(Y)river are main water supplies of the city of Wuhan.In the present study the effect of organic extracts of chlorinated drinking water(CDW)processed from raw water of D lake,H river and Y river on lipid peroxidation(LPO)was evaluated in human HepG2 cells to explore the possible mechanism of cell damage caused by CDW extracts.HepG2 cells were exposed in vitro to CDW extracts of concentrations corresponding to 0 167,1 67,16 7 and 167ml CDW/ml culture medium.As a product of LPO and an intracellular antioxidant,malondialdehyde(MDA)and glutathione(GSH)were determined in HepG2 cells after CDW extracts treatment.DMSO(7μl/ml culture)and H 2O 2(100μmol/L)were used as solvent control and positive control respectively.HepG2 cells exhibited significant increase of MDA levels(0 062±0 015,0 065±0 012 and 0 070±0 014μg/mg protein)after treatment of CDW extracts from D lake for 16 7 and 167ml CDW/ml culture and H river for 167ml CDW/ml culture in comparison with DMSO solvent control(0 029±0 003μg/mg protein).No significantly enhanced levels of MDA were observed in HepG2 cells exposed to CDW extracts from Y river.Additionally,all the concentrations of CDW extracts from D lake for 0 167,1 67,16 7 and 167ml CDW/ml culture lead to significant decreased GSH(0 048±0 006,0 043±0 003,0 042±0 001 and 0 042±0 006μg/mg protein)compared to the DMSO solvent control(0 062±0 001μg/mg protein).GSH decrease was also found at the higher concentrations of CDW extracts from H river and Y river for 16 7 and 167ml CDW/ml culture.Statistical analysis showed a inverse correlation between the levels of MDA and GSH in HepG2 cells after treatment of CDW extracts from D lake,Y river and H river.The results indicated that CDW extracts could have obvious LPO effect on HepG2 cells in vitro.CDW extracts from D lake showed stronger LPO effect than those from H river and Y river.

关 键 词：氯化饮水有机提取物 HEPG2细胞 脂质过氧化作用 肝肿瘤细胞 细胞损伤 丙二醛 还原型谷胱甘肽 遗传毒性 

分 类 号：R36[医药卫生—病理学] X824[医药卫生—基础医学]