胃癌组织中的肺耐药蛋白、P糖蛋白、谷胱甘肽-S-转移酶以及拓扑异构酶Ⅱ的表达及意义  

Expression and significance of LRP,P-gp,GST-π and TOPOⅡ in gastric carcinoma

作　　者：李海[1] 郑春宁[2] 薛强[1] 万厚民[1] 王震宇[1] 

机构地区：[1]泰山医学院第二附属医院普外科,济南250031 [2]济南市中心医院

出　　处：《中华实验外科杂志》2005年第1期58-59,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　探讨肺耐药蛋白 (LRP)、P糖蛋白 (P gp)、谷胱甘肽 S 转移酶 (GST π)以及拓扑异构酶Ⅱ (TopoⅡ )在胃癌组织中的表达及意义。 方法　采用免疫组织化学链霉菌抗生物素蛋白 过氧化酶连接法 (S P法 )对 71例术前未行化疗的胃癌组织切片中的LRP、P gp、GST π以及TopoⅡ的表达进行分析。结果　LRP、P gp、GST π以及TOPOⅡ的表达阳性率分别为5 3 .5 % ,69.0 % ,74.6% ,46.4%。与肿瘤患者的年龄、性别、肿瘤的发生部位和肿瘤的浸润深度无关。LRP、P gp、GST π表达与肿瘤的组织学类型有关 ,高分化腺癌、中分化腺癌、低分化腺癌和未分化癌之间比较差异有统计学意义 (χ2 =8.90 ,P <0 .0 5 ,χ2 =9.0 9,P <0 .0 5 ;χ2 =10 .198,P <0 .0 1)。LRP、P gp、GST π的表达与淋巴结转移有关 (χ2 =5 .90 7;P <0 .0 5 ;χ2 =5 .2 2 9,P <0 .0 5 )。结论　LRP、P gp、GST π以及TOPOⅡ的表达是肿瘤耐药的标志物 ,可作为肿瘤化疗时药物取舍的参考。LRP、P gp、GST π的表达与胃癌的组织学类型有关 ,LRP、GST π可能在胃癌转移中发挥了重要作用。Objective To study the expression and significance of lung resistance protein (LRP),P glycoprotein (P gp),glutathione S transferace π (GST π) and topoisomerase Ⅱ (TopoⅡ) in gastric carcinoma tissues.Methods Seventy one previoulsly untreated patients with human gastric carcinomas were studied for the expression of LRP,P gp,GST π and Topo Ⅱ by using immunohistochemistry.Results The positiveexpression rate of LRP,P gp,GST π and Topo Ⅱ was 53.5%, 69.0%, 74.6% and 46.4% in gastric carcinoma tissues.There was significant relationship between the LRP ( χ 2 = 8.90, P < 0.05), P gp ( χ 2 =9.09, P < 0.05), GST π ( χ 2 = 10.198, P < 0.01) and histological types,also between the LRP ( χ 2 = 5.907, P < 0.05), GST π ( χ 2 = 5.229, P < 0.05) and lymph node metastasis.Conclusion LRP,P gp,GST π and Topo Ⅱ are important tumor markers for tumor drug resistance.There is a correlationship between the expression ofLRP,P gp,GST π and histological types.LRP,GST π maybe play an important role during the lymph node metastasis of gastric carcinoma.

关 键 词：LRP P-gp GST-Π 表达 TopoⅡ 胃癌组织 拓扑异构酶Ⅱ 谷胱甘肽-S-转移酶 分化 链霉菌 

分 类 号：R735.2[医药卫生—肿瘤]