缺血预处理联合阿魏酸钠对大鼠脑缺血再灌注损伤的保护作用及其机制  

Protective mechanism of cerebral ischemic preconditioning combined with sodium ferulate on global ischemia reperfusion injury

作　　者：邓志锋[1] 李明[1] 汪泱[2] 宋书欣[1] 

机构地区：[1]江西医学院第二附属医院神经外科,南昌330006 [2]江西医学院第一附属医院泌尿外科研究所

出　　处：《中华实验外科杂志》2005年第1期95-97,共3页Chinese Journal of Experimental Surgery

基　　金：江西省自然科学基金资助项目 (0 0 4 0 0 37)

摘　　要：目的　探讨脑缺血预处理联合阿魏酸钠对脑缺血再灌注损伤的保护作用及其机制。方法　采用四血管阻断法复制全脑缺血模型 ,动物随机分为对照组、缺血预处理组、缺血预处理 +阿魏酸钠组和缺血组 ,采用免疫组织化学染色、TUNEL染色等方法观察各组动物在脑缺血再灌注后皮层和海马CA1区Fas蛋白、Caspase 8蛋白的表达、神经元数及凋亡细胞计数的情况。 结果　阿魏酸钠 +缺血预处理组凋亡细胞数较缺血组和缺血预处理组显著减少 ,缺血 7d时皮层及海马CA1区神经元无明显丢失 ,而缺血组神经元大量丢失。缺血预处理 +阿魏酸钠组Fas阳性细胞与缺血组相比显著减少 ,Caspase 8蛋白阳性细胞则较缺血预处理组和缺血组显著减少。 结论　缺血预处理联合阿魏酸钠可以进一步加强缺血预处理对脑缺血再灌注损伤的保护作用 ,其机制可能是通过减少脑缺血后神经元Caspase 8蛋白的表达 ,从而抑制细胞凋亡。Objective To explore the protective effects and the mechanism of cerebral ischemic preconditioning combined with sodium ferulate (SF) on global ischemia (I) reperufusion injury of rats.Methods Global cerebral ischemia was induced in rats by four vessel occlusion.Animals were allocated to the following groups before the experiment:sham (S);IP;SF+IP;and I.Nissl staining and terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick end labeling (TUNEL) were used to count the number of alive neurons and apoptotic cells in the cortex and hippocampal CA1 of rats.Immunohistochemistry with Fas protein polyclonal antibody and Caspase 8 protein polyclonal antibody were used to explore the changes in the Fas protein and Caspase 8 protein expression after global cerebral ischemia.Results The number of apoptotic cells in SF+IP group was less significantly than that in IP group and I group.The alive neurons in cortex and hippocampus in SF+IP group showed no significant changes,but those in I group were reduced remarkably after 7 days of global cerebral ischemia.The cells positive for Fas protein expression in SF+IP group were less than those in I group significantly,and the Caspase 8 protein expression cells in SF+IP group were less significantly than those in IP group and I group.Conclusion Ischemia preconditioning combined with sodium ferulate can enhance the effects of cerebral ischemic preconditioning on global ischemia reperufusion injury,possibly by reducing the expression of the expression of Caspase 8 and subsequently suppressing the apoptosis in cortex and hippocampal CA1 region induced by global ischemia.

关 键 词：缺血预处理 阿魏酸钠 脑缺血再灌注损伤 保护作用 联合 CASPASE-8蛋白 神经元 动物 凋亡细胞 表达 

分 类 号：R743[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]