p53基因第72位密码子多态与瘢痕疙瘩易感性研究  

p53 GENE CODON 72 POLYMORPHISM AND SUSCEPTIBILITY TO KELOID IN CHINESE POPULATION

作　　者：卓阳[1] 高建华[1] 罗深秋[2] 曾位森[2] 胡志奇[1] 鲁峰[1] 赵永忠[2] 

机构地区：[1]南方医科大学(原第一军医大学)南方医院整形外科,广州510515 [2]南方医科大学细胞生物学教研室

出　　处：《中国修复重建外科杂志》2005年第1期28-30,共3页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：广东省科技计划资助项目 (2 0 0 2 C1 0 4 0 4 0 1 )~~

摘　　要：目的　探讨 p5 3基因第 72位密码子多态与瘢痕疙瘩遗传易感性的关系。　方法　采用聚合酶链反应 -反向斑点杂交、DNA直接测序方法 ,检测了 15例瘢痕疙瘩患者与 15名正常人对照的 p5 3基因第 72位密码子的基因型。　结果　瘢痕疙瘩组的 Pro等位基因及 Pro/ Pro基因型频率明显高于对照组 (P值分别为 0 .0 35、0 .0 30 ) ,Pro/Arg、Arg/ Arg基因型频率在病例组和对照组的分布差异无显著性。　结论　 p5 3基因第 72位密码子 Pro等位基因及Pro/ Pro基因型可能是瘢痕疙瘩的易感因素。Objective To investigate the relationship between p53 codon 72 polymorphism and susceptibility to keloid. Methods The p53 genotypes were detected by polymerase chain reaction reverse dot blot(PCR RDB) and DNA direct sequencing among 15 healthy controls and 15 patients with keloid. Results The frequency of the Proallele( P =0.035) and Pro/Pro genotype(P=0.030) in patients was significantly higher than that in the controlls. There was no significant difference in the frequency of Pro/Arg and Arg/Arg genotypes between patients and controls. Conclusion The p53 gene codon 72 polymorphism may play a role in susceptibility to keloid.

关 键 词：瘢痕疙瘩 p53基因 对照组 易感性 等位基因 易感因素 DNA直接测序 密码子 多态 聚合酶链反应 

分 类 号：R622[医药卫生—整形外科]