可见光照射致培养的视网膜色素上皮细胞凋亡的超微结构观察  

Observation on ultrastructure of cultured retinal pigment epithelium cells apoptosis induced by visible light

作　　者：宋忆淑[1] 贺冰[2] 费向东[3] 李玉新[1] 张晓光[4] 

机构地区：[1]东北师范大学遗传与细胞研究所,吉林长春130024 [2]吉林大学第一医院眼科 [3]长春市双阳区医院骨科 [4]吉林大学第二医院眼科

出　　处：《中国老年学杂志》2005年第1期69-70,共2页Chinese Journal of Gerontology

基　　金：吉林省科技厅自然科学基金资助项目(200305414);中国博士后科学基金资助(2004035557)

摘　　要：目的研究可见光照射导致培养的视网膜色素上皮(retinalpigmentepithelium,RPE)细胞凋亡的超微结构变化。方法以卤钨灯为光源,(1970±200)Lx的光照强度,持续光照30min为光损伤实验条件,以透射电镜观察为检测手段,观察可见光照致RPE细胞损伤的超微结构变化。结果光照后培养的RPE细胞,出现了细胞凋亡早期的特征性改变;在相同时间点,细胞表现了不同程度的损害;随着培养时间的延长,细胞出现损害的程度逐渐加重,受损的细胞数目逐渐增多。结论一定强度的可见光照射可导致培养的RPE细胞发生细胞凋亡的特征性超微结构改变,细胞受损具有非同步性及不可逆趋势。Objective To explore the ultrastructure change of the apoptosis of cultured retinal pigment epithelium ( RPE) cells induced by visible light. Methods Being the light source,(1 970±200)Lx tungsten lamp was used for 30 minutes to damage the PC12 cells. The ultrastructure change of cell injury induced by visible light was observed by transmission electron microscope. Results Earlier apoptosis characteristics were observed in cultured RPE cells that were damaged by light. With prolongation of culture time, the injury was deteriorated and the number of injured cell increased. Conclusions Visible light with certain strength can induce the characteristic ultrastructure change of apoptosis in cultured RPE cells without synchronism and reversibility.

关 键 词：光刺激 色素上皮 眼 超微结构 细胞凋亡 

分 类 号：R774[医药卫生—眼科]