糖尿病大鼠视网膜血管内皮细胞生长因子及碱性成纤维细胞生长因子的表达  

Expression of vascular endothelial growth factor and basic fibroblast growth factor in diabetic rats

作　　者：宋鄂[1] 董宇[1] 崔治华[1] 杨威[1] 王雅坤[2] 吴家祥[3] 

机构地区：[1]吉林大学第一医院眼科,长春130021 [2]延边大学医学院 [3]吉林大学基础医学部病理教研室

出　　处：《中华眼底病杂志》2005年第1期37-40,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：吉林省科学基金资助项目(9905755)

摘　　要：目的研究糖尿病大鼠视网膜病变早期血管内皮细胞生长因子(VEGF)及碱性成纤维细胞生长因子(bFGF)作用的关系。方法Wistar大鼠55只,建立糖尿病大鼠模型,随机分为正常对照组(10只),糖尿病1、3、5个月组(每组各15只)。每组分别在视网膜石蜡切片上行VEGF、bFGF原位杂交及免疫组织化学检测,以观察两者的表达情况。结果原位杂交检测结果显示,bFGF在糖尿病3个月组开始有表达,表达个体数百分比为77.8%,5个月组表达个体数百分比为88.9%;VEGF在糖尿病3个月组无阳性表达,5个月组表达个体数百分比为66.7%。免疫组织化学检测结果显示,bFGF在糖尿病3个月组开始阳性表达,表达个体数百分比为55.6%,5个月组表达个体数百分比为88.9%;VEGF在3个月组表达个体数百分比为33.3%,5个月组表达个体数百分比为88.9%。结论糖尿病大鼠视网膜病变的VEGF表达出现在bFGF之后。Objective To investigate the expression and relation of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in rats with diabetic retinopathy. Method Fifty-five Wistar rats were randomly divided into the control group (10 rats), and 1, 3, and 5-month-diabetes group (15 rats in each diabetes group), and the diabetic models were set up. The expressions of VEGF and bFGF were detected by situ hybridation and immunohistochemistry on retinal paraffin sections. Results The results of situ hybridation showed that expression of bFGF was found in 3-month-deatbtes group with the percentage of 77.8%, and 88.9% in 5-month-deatbtes group; the positive expression of VEGF was not found in 3-month-deatbtes group but in 5-month-deatbtes group with the percentage of 66.7%. Immunohistochemistry indicated that the positive expression of bFGF started in 3-month-deatbtes group with the percentage of 55.6%, and 88.9% in 5-month-deatbtes group; the percentage of the expression of VEGF was 33.3% in 3-month-deatbtes group and 88.9% in 5-month-deatbtes group. Conclusion The expression of VEGF occurs after the expression of bFGF in rats with DR.

关 键 词：阳性表达 糖尿病大鼠 FGF VEGF 免疫组织化学检测 碱性成纤维细胞生长因子 视网膜病变 百分比 结论 目的 

分 类 号：R774.1[医药卫生—眼科]