仙台病毒HN基因核酸疫苗的构建及初步鉴定  

Construction and Primary Identification of DNA Vaccine Harboring HN Gene of Sendai Virus

作　　者：张国际[1] 李晓眠[1] 佟惠春[1] 

机构地区：[1]天津医科大学微生物学教研室,300070

出　　处：《天津医药》2005年第1期17-19,共3页Tianjin Medical Journal

摘　　要：目的:构建用于防治仙台病毒感染的核酸疫苗并初步鉴定。方法:使用逆转录-聚合酶链反应(RT鄄PCR),从新分离的仙台病毒天津株基因组中扩增出HN全部编码序列,将其插入pcDNA3的Pcmv下游,构建仙台病毒HN基因核酸疫苗。PCR、酶切和测序进行鉴定。结果:鉴定证实了核酸疫苗的构建。结论:构建好的核酸疫苗为下一步的体外表达和动物免疫实验奠定基础。Objective: A specific DNA vaccine has been constructed in order to prevent human being from parainfluenza family infection. Methods: HN-coding sequence has been amplified from new isolated Sendai virus, tianjin strain using RT-PCR, and inserted into the downstream of Pcmv of vector pcDNA3, therefore, DNA vaccine harboring HN gene of Sendai virus is constructed. The recombinator is well identified by series of methods such as PCR, RE digestion and sequencing. Results: The construction has been successfully verified by the methods mentioned above. Conclusion: The recombinator will provide framework for its expression in vitro and animal immunological test in the future.

关 键 词：核酸疫苗 仙台病毒 鉴定 HN基因 构建 逆转录一聚合酶链反应 PCDNA3 结论 目的 基础 

分 类 号：R730[医药卫生—肿瘤] R392[医药卫生—临床医学]