基因扩增技术检测急慢性B—淋巴细胞白血病免疫球蛋白基因重排  

DETECTION OF GENE REARRANGEMENT OF IMMUNOGLOBULIN IN ACUTE AND CHRONIC LYMPHOID LEU KEMIAS OF B-CELL LINEAGE

作　　者：董作仁[1] 李栋梁[1] 徐世荣[1] 潘凌[1] 张瑞欣[1] 李贵彬[1] 姜玲玲[2] 温进坤[2] 魏素珍[2] 姚尔固[2] 

机构地区：[1]河北医学院二院,050000 [2]河北医学院基础所

出　　处：《山西白血病》1993年第1期16-18,共3页

摘　　要：应用聚合酶链反应(PCR)扩增IgH重排产生的CDR—Ⅲ序列,检测了23例急慢性B—淋巴细胞白血病患者,20例发生IgH重排,其中4例出现两种基因扩增产物。6例完全缓解的患者,3例检出异常重排的IgH基因,并先后复发。本文还用Southern blot法与PCR进行了对照性研究。Polymerase chain reaction (PCR) was used to amplify the complementarity determining region 3 (CDR--3) of immunoglobulin gene heavy (IgH). Among 23 cases of ALL and CLL patients detected, IgH rearrangment occured in 20 cases, and 2 kinds of gene amplified prod- ucts appeared in 4 of them. Among 6 complete remission patients, abnormal rearranged IgH gene was detected in 3 of them successively. We had also done control research between southern blot method and PCR technique.

关 键 词：白血病 聚合酶链反应 免疫球蛋白 

分 类 号：R733.7[医药卫生—肿瘤]