乙醇激活对猪体外成熟卵母细胞发育的影响  

Effects of Ethanol Activation on the Rates of Cleavage and Blastocyst of Porcine IVM Oocytes

作　　者：田见晖[1] 朱士恩[1] 曾申明[1] 刘国世[1] 蔡元[2] 刘焕营[1] 李迎[1] 张忠诚[1] 

机构地区：[1]中国农业大学动物科技学院,北京100094 [2]甘肃农业大学动物科技学院,甘肃兰州730070

出　　处：《中国兽医学报》2005年第1期99-101,共3页Chinese Journal of Veterinary Science

基　　金：北京市科委重点项目 (H0 2 2 0 2 0 0 60 42 0 ) ;高校博士点基金项目 (2 0 0 2 0 0 190 3 1)

摘　　要：探索了单独应用乙醇或者联合 6 -氨基嘌呤 (DMAP)、细胞松弛素 B(CB)、放线菌酮 (CHX)等化学物质对体外成熟 4 4 h猪卵母细胞激活的影响。结果如下 :分别用 0、5 %、8%、11%乙醇处理 5 min,或者在 8%乙醇条件下激活处理2、5、10 min的各组试验中 ,8%乙醇处理 5 min的卵裂率为 (6 .4 2± 4 .88) % ,高于其他各组 ,但均无囊胚发育 ;乙醇(8%、5 min)激活后 ,分别用 CB、CHX、DMAP、CB+CHX、CB+DMAP培养 6 h,结果 CB+DMAP的激活率、卵裂率和囊胚率最高 ,分别为 (70 .86± 3.75 ) %、(5 3.87± 5 .36 ) %、(15 .0 7± 5 .5 3) % ,与其他组相比 ,囊胚率差异显著 (P<0 .0 5 )Effects of ethanol and combined with cytochalasin B(CB) or cycloheximide(CHX) or 6-dimethylaminopurine(DMAP) on the activation of porcine oocytes at 44 h of maturation in vitro were investigated.In this experiment,the development rates of ethanol-induced parthenogenetic activation of oocytes were compared.No blastocysts developed when ethanol alone at various concentrations(5%-11%) were used for 2 to 10 minutes.For the combination methods,the activation rate of oocytes was (70.86±3.75)% when exposed to 8% of ethanol for 5 min and treated in activation medium containing CB and DMAP for 6 h,and (15.07±5.53)% of parthenotes developed to blastocyst stage,higher(P<0.05) than the other combined treatments.

关 键 词：激活 CB 乙醇 卵裂率 单独应用 影响 联合 囊胚率 DMAP 体外成熟 

分 类 号：R743.32[医药卫生—神经病学与精神病学] S823[医药卫生—临床医学]