胃癌中ZNF217基因扩增的研究  

Study on amplification of ZNF217 in primary gastric carcinoma

作　　者：朱亚青[1] 朱正纲[1] 刘炳亚[1] 陈雪华[1] 张奕[1] 尹浩然[1] 

机构地区：[1]上海第二医科大学附属瑞金医院外科上海消化外科研究所

出　　处：《中华胃肠外科杂志》2005年第1期60-62,共3页Chinese Journal of Gastrointestinal Surgery

基　　金：上海市医学领先专业(胃肠道肿瘤生物学特性及综合治疗的基础与临床研究)基金资助项目(98-Ⅲ-005);上海市实体瘤攻关项目(98-ZD-001)

摘　　要：目的探讨原发性胃癌中ZNF217(zincfingerprotein217)基因扩增情况及其与胃癌临床病理学特征之间的关系。方法用半定量PCR方法检测47例胃癌中ZNF217基因DNA拷贝数变化情况。结果ZNF217基因相对拷贝数在胃癌和正常胃黏膜中比较,差异无统计学意义(t=0.900,P=0.373)。原发性胃癌中ZNF217基因扩增率为11.36%,ZNF217基因扩增多见于肿瘤直径大于或等于5cm的胃癌(P<0.01)和肠型胃癌(P<0.05)。结论ZNF217癌基因可能仅在部分胃癌中发挥作用,20q13上还存在其他的胃癌相关癌基因。Objective To investigate amplification of zinc finger protein 217(ZNF217) and its association with clinicopathologic parameters in primary gastric carcinoma. Methods Semiquantitative polymerase chain reaction(PCR) was used to determine DNA copies of ZNF217 in the specimens from forty seven cases with primary gastric carcinoma. Results There was no difference in DNA copies between tumor specimens and paratumor normal tissues. The incidence of ZNF217 amplification was 11.36%in gastric cancer. The amplification of ZNF217 was significantly associated with tumor size(P< 0.01) and intestinal type of stomach cancer(P< 0.05). Conclusions Oncogene ZNF217 may play a role in specific tumor types or subtypes of gastric cancer. There may be other oncogenes associated with gastric carcinoma in 20q13.

关 键 词：原发性胃癌 基因扩增 癌基因 拷贝数 临床病理学特征 肠型胃癌 正常 DNA PCR方法 

分 类 号：R735.2[医药卫生—肿瘤] R737[医药卫生—临床医学]