Dot-PPA-ELISA检测仔猪副伤寒血清抗体方法的研究  

Dot-PPA-ELISA method for the detection of antibody against paratyphus swine

作　　者：黄艳艳[1] 曹三杰[1] 文心田[1] 

机构地区：[1]四川农业大学动物科技学院预防医学系,四川雅安625014

出　　处：《中国预防兽医学报》2005年第1期63-66,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：以沙门氏菌的改良H E 抗原为膜载抗原 ,建立了检测仔猪副伤寒血清抗体的Dot_PPA_ELISA方法。研究选取了特异性良好的抗原 ,确定了Dot_PPA_ELISA的最佳工作条件 ,初步确定了感染仔猪副伤寒的Dot_PPA_ELISA抗体阳性标准为 1∶32。本研究制备的诊断膜片特异性强、敏感性高 ,能够检测到 1∶2 0 4 8稀释的动物试验阳性血清 ;该方法操作简便快捷、结果客观、易于判定、各种试剂材料均可做到标准化 ,适合规模化养猪场仔猪副伤寒的抗体监测以及流行病学调查之用。A Dot-PPA-ELISA method has been developed for the detection of anti-Salmonella antibody in pigs.Following work has been done for the establishment of the antibody detection method.Different kinds of Salmonella antigens have been tried and an improved heat-extracted antigen was used as the diaphragm antigen.The optimum condition of Dot-PPA-ELISA was determined.The antibody positive standard of Salmonella infection was established as 1∶32.The high specificity of Dot-PPA-ELISA was proved by the specific blocking test,and also by the cross-reaction test in which the diaphragm didn't react with the antibodies against Pasteurellosis,Streptococcosis,Colibacillosis,Chlamydiosis,HCV,PPV,Brucellosis,PRV and foot and mouth disease.The diaphragm has good sensitivity and could detect some Salmonella-positive test serum which has been diluted to 1∶2048.Store at 4?℃ for at least 6 months or at 10?℃～25?℃ for 4 months,the sensitivity and specificity of the diaphragm didn't change,so it has good stability.This study indicates that the Dot-PPA-ELISA was a convenient,rapid,sensitive and specific method for the detection of Salmonella antibody.Otherwise,all of the materials used in Dot-PPA-ELISA can be standardized,so it's fit for the surveillance of Salmonella antibody in herd and for the epidemiologic investigation of paratyphus swine.

关 键 词：DOT-PPA-ELISA 仔猪副伤寒 抗体监测 

分 类 号：S852.51[农业科学—基础兽医学]