四种试剂盒在SARS实验室早期诊断中的作用分析  

The effects of 4 laboratory test kits in early detecting of severe acute respiratory syndrome coronavirus

作　　者：王鸣[1] 高阳[1] 周端华[1] 吴新伟[1] 陈小霜[1] 狄飚[1] 刘于飞[1] 陈芳[1] 杜琳[1] 徐慧芳[1] 顾菁[1] 郑伯健[2] 徐建国[3] 

机构地区：[1]广州市疾病预防控制中心,510080 [2]香港大学微生物系 [3]中国疾病预防控制中心传染病预防控制所

出　　处：《中华流行病学杂志》2005年第1期22-24,共3页Chinese Journal of Epidemiology

基　　金：广东省科技厅资助项目 (2 0 0 3FD0 2 0 4)

摘　　要：目的　比较 4种检测严重急性呼吸综合征冠状病毒 (SARS CoV)基因、抗原和抗体的试剂盒在SARS患者早期实验室诊断的可能作用。方法　用 3种酶联免疫吸附试验 (ELISA)分别检测SARS CoVIgG、SARS CoVIgM 和SARS CoVN蛋白 ,用荧光定量聚合酶链反应 (F PCR)检测SARS CoV核酸。结果　根据 16 2份血清的检测结果 ,发病 1- 5天 ,N抗原的阳性率可达 90 .2 % (5 5 /6 1) ;发病后的第 15 - 18天IgG和IgM阳性率为92 .8% (13/ 14 ) ;根据 82份咽拭子的检测结果 ,F PCR法在发病后 1- 5天的阳性率可达 5 6 .3% (14 / 2 4 ) ,6 - 9天可达 71.4 % (10 / 14 )。结论　除了检测SARS CoV病毒基因的F PCR方法以外 ,对疑似患者血清标本进行N蛋白的检测 ,具有早期预报意义。Objective To compare the 4 test kits on severe acute respiratory syndrome coronavirus (SARS-CoV) gene, antigen and antibody for early diagnose of SARS patients. Methods Three enzyme linked immunosorbent assay (ELISA) kits were used to detect SARS-CoV IgG, IgM and N protein and fluorescent polymerase chain reaction (F-PCR) kit was used to detect SARS-CoV RNA. Results In 162 serum samples, 90.2%(55/61) became N protein positive in 1-5 days and 92.8%(13/14) became positive IgM and IgG in 15-18 days after the onset of disease, respectively. On 82 gorgling samples, the positive rates of F-PCR were 56.3%(14/24) in 1-5 days and 71.4%(10/14) in 6-9 days after the onset. Conclusion Other than F-PCR, N protein had good effect in the early detection on dubious patients which could lead to effective prevention and control of the epidemic.

关 键 词：SARS-COV 发病 病后 室早 试剂盒 抗原 阳性率 N蛋白 PCR方法 预报 

分 类 号：R511.9[医药卫生—内科学]