槲皮素对LPS延迟中性粒细胞自发性凋亡效应的抑制作用  

Inhibitory effect of quercetin on lipopolysaccharide-induced delay in spontaneous apoptosis of neutrophils

作　　者：宋传旺[1] 刘佳佳[1] 段承刚[1] 谢华福[1] 甘淋[1] 刘晓燕[1] 何涛[1] 何延政[1] 

机构地区：[1]泸州医学院中心实验室,泸州646000

出　　处：《中国免疫学杂志》2005年第1期13-16,共4页Chinese Journal of Immunology

基　　金：四川省杰出青年基金资助项目 (川科基 [2 0 0 1] 2 #);泸州市科技局重点资助项目 (泸市科 [2 0 0 2 ] 71#)

摘　　要：目的 :研究槲皮素对接受细菌脂多糖 (LPS)刺激的中性粒细胞 (PMN)自发性凋亡的影响 ,探讨槲皮素的抗炎作用机制。方法 :以人外周血PMN为研究对象 ,选择光镜和流式细胞术检测细胞凋亡情况 ,对裂解的片段化DNA进行定量 (二苯胺测定法 )和定性 (琼脂糖凝胶电泳 )分析。结果 :槲皮素 (1～ 10 0 μmol/L)对PMN的自发性凋亡率并无影响 ,但却能部分恢复由LPS所延迟的PMN自发性凋亡进程。当槲皮素的浓度为 4 0 μmol/L时 ,其恢复作用达到最大。 结论 :槲皮素对LPS延迟PMN自发性凋亡的效应产生了抑制作用 ,减轻了因预激因子活化PMN而加重的炎症反应 ,部分揭示了槲皮素的抗炎作用机制。Objective:To assess the regulatory effect of quercetin on the inflammation,the role of quercetin in the spontaneous apoptosis of neutrophils treated with of lipopolysaccharide (LPS) was investigated.Methods:Apoptosis was determined by light microscopy and flow cytometry.Quantitation and qualitative analysis of DNA fragmentation were performed by diphenylamine assay and agarose gel electrophoresis.Results:Quercetin (1～100 μmol/L) failed to affect the spontaneous apoptosis of neutrophils,but it partly restored LPS delayed neutrophil apoptosis.The concentration of quercetin required to reach a maximum restoring effect was approximately 40 μmol/L.Conclusion:Quercetin prevent LPS induced delay in neutrophil spontaneous apoptosis,which would diminish inflammatory response mediated by LPS primed neutrophils.This could partly explain the anti inflammatory effect of quercetin. [

关 键 词：中性粒细胞 凋亡 槲皮素 细菌脂多糖 炎症 

分 类 号：R361.3[医药卫生—病理学]