生物型人工肝的在线快速细胞监测系统  

Online rapid monitoring system for cell viability and function in bioartificial liver support system

作　　者：项红升[1] 刘剑峰[1] 李明[1] 霍荣岭[1] 

机构地区：[1]中国科学院研究生院,电工研究所,北京100080

出　　处：《生物医学工程与临床》2005年第1期45-48,共4页Biomedical Engineering and Clinical Medicine

摘　　要：目的在不破坏生物反应器内细胞生存环境的条件下,在线快速了解反应器内肝细胞的生存状态、功能活性,以便及时采取相应的控制措施。方法改变传统的大型分析仪器离线检测的方法,采用以单色光源、集成光电传感器为测量元件的单片机控制的专用监测系统,实现了对白蛋白、尿素的在线快速检测。结果实验证明,该系统结构新颖简单,能够满足生物型人工肝系统的细胞功能活性检测任务。结论该方案实际可行,为生物型人工肝的开发评价提供了及时的依据,从而使生物型人工肝脏的闭环控制实现成为可能。Objective To develop an online cell monitoring system for rapid understanding the cell viability and functional activity- hence the appropriate measures could be adopted in time. Methods The traditional off line technique was changed into online monitoring in this instrument for rapid detecting albumin and urea. The monocolor light source and integrated photoelectric sensor were adopted in this system. Results It was demonstrated in experiment that this system was designed with a novel and simple construction,and it could fulfil the requirements of measuring the cell functional activity in bioartificial liver. Conclusion This design is feasible and reliable. It provides a novel system to monitor cell viability and function online quickly.Thus using this monitoring system,the closed-loop controlling of bioartificial liver support system can be realized.

关 键 词：生物型人工肝 肝细胞 功能活性 白蛋白 改变 细胞功能 肝脏 结论 实际 任务 

分 类 号：R318.6[医药卫生—生物医学工程]