木黄酮对白血病K562细胞增殖的影响  

Inhibitive effects of genistein on proliferation of leukemia K562 cells

作　　者：张敬东[1] 侯克佐[1] 罗颖[1] 刘世洲[1] 王甦[1] 刘云鹏[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001

出　　处：《中国医科大学学报》2004年第6期522-524,共3页Journal of China Medical University

摘　　要：目的探讨木黄酮(genistein)抑制白血病K562细胞增殖的机制。方法用噻唑蓝染色法检测genistein对K562细胞增殖的抑制作用;用流式细胞仪进行细胞周期解析,通过细胞形态学观察细胞分裂;应用RTPCR方法检测白血病细胞WT1mRNA表达。结果Genistein浓度分别为2～30μg/ml作用K562细胞72h,细胞增殖呈浓度依赖性受抑,与对照组相比差异显著(P<0.01),IC50=11.4μg/ml;细胞周期解析发现,浓度为30μg/ml的genistein作用K562细胞12h,引起了明显的G2/M期阻滞,24h后G2/M期细胞增至93%;细胞分裂指数与对照组比没有明显差别;30μg/mlgenistein作用K562细胞24h,3h起WT1mRNA表达开始降低,24h时降至对照的30%。结论genistein抑制K562细胞增殖,诱导G2期或M早期阻滞及下调WT1的表达。Objective: To investigate the effects of genistein on proliferation of leukemia K562 cells. Methods: The cell growth was measured by using MTT assay. Cell cycle was analyzed by using flow cytometry and cells morphology. Expression of WT1 mRNA was detected by using reverse transcription-polymerase chain reaction (RT-PCR). Results: After exposed to 2 to 30 μg/ml of genistein for 72 hours, proliferation of K562 cells were inhibited in a concentration-dependent manner (P<0.01), and IC 50 was 11.4 μg/ml. Genistein (30 μg/ml) induced a distinctive G 2 /M phase arrest at 12 hours, the percent of cells in G 2 /M phase reached 93% at 24 hours, but mitosis index showed no difference with control. Genistein (30μg/ml) decreased the expression of WT1 mRNA in K562 cells from the 3rd hour and reached 30% of the control at 24 hours.Conclusion: Genistein can inhibit the proliferation of K562 cells ,induce G 2 or early M phase arrest, and decrease the expression of WT1mRNA.

关 键 词：木黄酮 白血病 细胞周期 基因 

分 类 号：R733.7[医药卫生—肿瘤]