Bcl-2、C-erbB-2基因与卵巢肿瘤的关系  

Relationship between expressions of bcl-2、c-erbB-2 gene and ovarian tumors

作　　者：杜丹丽[1] 汪万英[2] 赵发英[1] 姚敏[3] 

机构地区：[1]蚌埠医学院附属医院妇产科,安徽蚌埠233004 [2]蚌埠医学院附属医院病理科 [3]蚌埠医学院病理教研室

出　　处：《中国微生态学杂志》2004年第4期198-200,共3页Chinese Journal of Microecology

基　　金：安徽省教委自然科学基金资助课题 ( 2 0 0 2KJ2 2 6)

摘　　要：目的　探讨bcl 2凋亡抑制基因、c erbB 2癌基因和细菌L型感染在卵巢肿瘤中的表达及其意义。方法　采用免疫组化和革兰染色方法 ,检测了 10 1例卵巢肿瘤中的bcl 2、c erbB 2基因的表达 ,以及细菌L型的检出率。结果　恶性肿瘤中Bcl 2、C erbB 2蛋白产物的表达率明显高于良性肿瘤 (P <0 0 1) ,Bcl 2、C erbB 2蛋白在卵巢恶性肿瘤中临床分期Ⅲ、Ⅳ期中的阳性表达率 ,明显高于Ⅰ、Ⅱ期 ,病理分级Ⅲ级的表达率明显高于Ⅰ、Ⅱ级 (P <0 0 1)。细菌L型的检出率与腹腔淋巴结有转移、有腹水者比无转移及无腹水者差异有显著性 (P <0 0 5 ) ,而细菌L型的检出率与肿瘤的类型、临床分期、病理分级差异无显著性 (P >0 0 5 )。结论　bcl 2、c erbB 2基因在卵巢肿瘤中有不同程度异常表达 ,两种基因的表达与肿瘤的临床分期及病理分级有明显相关性 (P <0 0 1)。L型感染极有可能导致基因的变异 ,因此L型感染可能成为诱发肿瘤形成的因素之一。bcl 2凋亡抑制基因、c erbB 2癌基因的过度表达和变异 ,以及细菌L型感染可能相互协同在卵巢肿瘤发生和发展过程中起重要作用。Objective To investigate expressions and the clinical significance of bcl-2 gene?c-erbB-2 gene,infection of L-forms bacterial in ovarian tumor.Methods Immunohistochemical straining was used to detect the expression of bcl-2 gene and c-erbB-2 gene in 101 ovarian tumor cases.Gram's straining was used to examine the positive rate bacterial L-forms in those cases.Results The expression of bcl-2 gene and c-erbB-2 gene was significantly higher in malignant tumors than in benign( P <0.01);The positive rate of expression of Bcl-2,C-erbB-2 protein in ovarian malignant tumors was higher in clinical stage Ⅲ and Ⅳ than in Ⅰ and Ⅱ.The positive rate of expression of bcl-2,c-erbB-2 pathological grades was higher in ovarian malignant tumors pathological stage Ⅲgrades than thatⅠ and Ⅱ grades( P <0.01).The significant difference was found between those cases that have lymph node metastases and ascites than that which have no ones( P <0.05).The positive rate of bacterial L-forms was no significant difference in tumor type?clinical stage and pathological grades( P >0.05).Conclusions bcl-2 gene and c-erbB-2 gene had certain positive expression in ovarian neoplasms.The expression of the two genes was obvious relative with clinical staging and pathological grades( P <0.01).Infection of bacterial L-forms was probably one of the inductive factors in the occurrence of tumor.Over-expression and variation of bcl-2 gene and c-erbB-2 gene and infection of L-forms bacterial was probably cooperative each other in the occurrence and development of ovarian tumors.

关 键 词：Bcl-2 卵巢肿瘤 C-ERBB-2基因 细菌L型 病理分级 临床分期 检出率 表达率 凋亡抑制基因 变异 

分 类 号：R737[医药卫生—肿瘤] R735[医药卫生—临床医学]