电离测量法^(14)C-尿素呼气试验诊断幽门螺杆菌的感染  

A novel ^(14)C-urea breath test with Geiger-Muller counters for rapid detection of Helicobacter pylori

作　　者：林木贤 张厚德 高燕 杜冀晖 麦丽文 朱丽娟 曾忠铭 

机构地区：[1]深圳市南山人民医院消化内科,广东深圳518052

出　　处：《中国微生态学杂志》2004年第4期215-216,共2页Chinese Journal of Microecology

摘　　要：目的　建立一种电离测量法14 C 尿素呼气试验 ,用于快速检测幽门螺杆菌感染。方法　口服14 C 尿素胶囊后 ,呼气14 CO2 直接采集于Ca(OH) 2 干粉垫上 ,14 C吸收量以双盖勒计数检测。结果与经典液体闪烁测量法比较。 81例幽门螺杆菌阳性和 10 2例阴性病人接受验证试验。结果　电离测量法诊断的准确性为略低于液体闪烁测量法的 ,但统计学差异无显著性 (92 .3%和 96 .2 % ,P >0 .0 5 ) ,标本重复测试结论变更率略高于液体闪烁测量 ,差异无显著性 (6 .0 %和 2 .2 % ,P >0 .0 5 )。结论　电离测量法14 C 尿素呼气试验可用于医生办公室现场快速幽门螺杆菌感染诊断。Objective To develop a novel 14 C-urea breath test (UBT) with Geiger-Muller counters for detection of H. pylori infection.Methods After intake of encapsulated 14 C-urea, exhaled 14 CO_2 in breath was absorbed to Ca(OH)_2-soaked pads on a dry cover surface .The amount of absorbed 14 C was detected using two Geiger-Muller counters operating in parallel . Results were compared with conventional UBT using liquids scintillation counting. 81 patients with H. pylori infection and 102 patients without infection were validated.Results The accuracy of novel 14 C-UBT was 92.3%, with 92.6% of sensitivity and 92.2% of specificity respectively; While the accuracy of conventional 14 C-UBT was 96.2% , with 97.5% of sensitivity and 95.1% of specificity respectively. There was no statistics significant difference between two methods.Conclusion The novel 14 C-UBT was suitable for rapid Helicobacter pylori detection and in-office analysis.

关 键 词：诊断 ^14C-尿素呼气试验 测量法 幽门螺杆菌感染 幽门螺杆菌阳性 阴性 病人 吸收量 采集 干粉 

分 类 号：R573[医药卫生—消化系统] R57[医药卫生—内科学]