变形链球菌对不同牙科充填材料的粘附及早期生物膜的形成  

Adhesion and early formation of Streptococcus mutans biofilm on various dental restorative materials

作　　者：张筱薇[1] 王扬[1] 张强[2] 高宁[3] 

机构地区：[1]深圳市第二人民医院口腔中心,广东深圳518035 [2]深圳市人民医院口腔中心,广东深圳518000 [3]四川大学华西口腔医学院,四川成都610041

出　　处：《中国微生态学杂志》2004年第4期224-225,227,共3页Chinese Journal of Microecology

基　　金：深圳市卫生科技项目 ( 2 0 0 2 0 40 69)

摘　　要：目的　探讨变形链球菌对不同牙科充填材料的粘附和早期生物膜的形成。方法　比较经放射性同位素3 H TDR(3 H 胸腺嘧啶核苷 )标记的变形链球菌对 3种唾液包被的充填材料的粘附。采用蛋白质测量试剂盒定量分析其对唾液蛋白的吸附量 ;采用凝胶电泳和图像分析系统定量分析其对唾液白蛋白和α 淀粉酶的吸收率。结果　各种材料对变形链球菌的粘附能力 ,对唾液蛋白的吸附能力均随着材料的不同而不同。FujiIX对细菌的粘附量很高 ,但是对蛋白的吸附量却很低 ;而F2 0 0 0对细菌的粘附量很低 ,对蛋白的吸附量却很高。结论　在不同充填材料表面形成的生物膜是不同的 ,提示早期生物膜的形成具有一定的特异性。这种生物膜的差异对口腔微生态环境及龋病和 /或牙周病的发展具有重要意义。Objective To examine the adhesion and formation of dental biofilm by Streptococcus mutans on various dental restorative materials.Methods The adhesion to the three saliva-coated retorative materials by 3H-thymidine-labelled Streptococcus mutans were compared.The amount of salivary proteins adsorbed onto the restorative materials was measured using protein assay kit.The percent of adsorbed amylase and albumin were quantified by gel electrophoresis and image evaluation system.Results The adhesion of Streptococcus mutans depended on the restorative materials.The same was the amount of adsorbed proteins.Fuji IX had the highest bacterial accumoulation,while showing the lowest affinity to amylase and albumin.F2000 demonstrated the highest affinity to amylase and albumin,while having the lowest bacterial accumulation.Conclusion Biofilm formation on various restorative materials differs,indicating that the initial biofilm accumulation on the material was specific to some degree.Such variation in biofilm formation had a significant impact on oral ecology and the progress of dental caries and/or periodontitis.

关 键 词：变形链球菌 粘附 充填材料 早期 牙科 唾液蛋白 生物膜 吸附量 吸附能力 环境 

分 类 号：R781[医药卫生—口腔医学] R780[医药卫生—临床医学]