注塑型聚甲基丙烯酸甲酯多通道微流控芯片的研制及其性能考察  

Microfabrication and Performance of Poly(methyl methacrylate) Microfluidic Chip with Multi-channels

作　　者：周小棉[1] 戴忠鹏[1] 罗勇[1] 刘大渔[1] 王辉[1] 毛秀丽[1] 林炳承[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023

出　　处：《高等学校化学学报》2005年第1期52-54,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20275039);国家自然科学基金重点项目(批准号:20035010);国家自然科学基金重大项目(批准号:20299030);中国科学院仪器研制专项基金资助

摘　　要：The microchip capillary electrophoresis devices were fabricated by using poly(methyl methacrylate)(PMMA) plastic material via an injection-molding process. The molded devices were enclosed by utilizing a mixed organic solvent to another PMMA film. The channel structure was very well defined and the molded channel surfaces were very smooth. The transmissivity was in the range from 91% to 93%(at the wavelength of 400—1 000 nm). In comparison to glass microchannels, the electroosmotic flow(EOF) in native PMMA channels was low. DNA marker separation was demonstrated in these PMMA devices with a high-resolution separation of double-stranded DNA fragments, chip-to-chip and the run-to-run reproducibility was good, and the relative standard deviation(%) values were below 2.2% for run-to-run data and 2.3% for the chip-to-chip comparisons. The PCR amplification products and proteins were analyzed on the PMMA chips. Such devices lead to the production of low-cost, disposable chips suitable for a variety of separation applications, including DNA sizing, DNA sequencing, protein and medical analysis. The detection limits of Rhodamine 6G dye for the unmodified PMMA chip and the modified PMMA chips were 1.0×10 -10 and 6.67×10 -13 mol/L, respectively.The microchip capillary electrophoresis devices were fabricated by using poly(methyl methacrylate)(PMMA) plastic material via an injection-molding process. The molded devices were enclosed by utilizing a mixed organic solvent to another PMMA film. The channel structure was very well defined and the molded channel surfaces were very smooth. The transmissivity was in the range from 91% to 93%(at the wavelength of 400—1 000 nm). In comparison to glass microchannels, the electroosmotic flow(EOF) in native PMMA channels was low. DNA marker separation was demonstrated in these PMMA devices with a high-resolution separation of double-stranded DNA fragments, chip-to-chip and the run-to-run reproducibility was good, and the relative standard deviation(%) values were below 2.2% for run-to-run data and 2.3% for the chip-to-chip comparisons. The PCR amplification products and proteins were analyzed on the PMMA chips. Such devices lead to the production of low-cost, disposable chips suitable for a variety of separation applications, including DNA sizing, DNA sequencing, protein and medical analysis. The detection limits of Rhodamine 6G dye for the unmodified PMMA chip and the modified PMMA chips were 1.0×10 -10 and 6.67×10 -13 mol/L, respectively.

关 键 词：微流控塑料芯片 毛细管电泳芯片 微加工 

分 类 号：O657[理学—分析化学]