丹参在常氧、急性低氧及氧反常等条件下对心室肌细胞L-Ca电流的影响  

Effects of salvia miltiorrhizae on L-Ca current in ventricular myocyte of guinea pig during normoxia, acute hypoxia and reoxygenation

作　　者：陈波[1] 马季骅[1] 张培华[1] 

机构地区：[1]武汉科技大学医学院,湖北武汉430080

出　　处：《中国病理生理杂志》2005年第1期85-88,共4页Chinese Journal of Pathophysiology

摘　　要：目的用不同浓度的丹参NaCl溶液作用于常氧、低氧、及氧反常的豚鼠心室肌细胞上,观察L-Ca通道电流的相对变化,以期解释丹参减轻及阻止细胞内钙超载的机理。方法使用全细胞膜片钳技术研究心室肌细胞L-Ca通道电流的变化。结果无论是常氧、缺氧和缺氧后复氧状态下,浓度为32、320、3200mg/L的丹参制剂都能有效降低L-Ca通道电流幅值并呈浓度依赖性。此外,低浓度32mg/L的丹参液对缺氧和氧反常细胞的作用更大于常氧细胞。结论丹参溶液能有效降低低氧和氧反常造成的异常增大的L-Ca电流幅值,阻止钙超载发生。AIM: To observe the changes of Ca-L current (ICa-L) and to investigate the mechanism of salvia miltiorrhizae (SM) for eliminating Ca^2+ overloaded in cells during acute hypoxia/reoxygenation. METHODS: The whole cell patch clamp technique was applied to study the changes of ICa-L. Different concentrations (32, 320, ~3 200 mg/L) of SM were added to the ventricular myocytes isolated from guinea pigs by enzyme digestion. RESULTS: SM (32, 320, ~3 200 mg/L) decreased the amplitude of ICa-L in a concentration-dependent manner regardless of these cells were under normoxia, hypoxia or reoxygenation. Furthermore, SM at low concentration (32 mg/L) was more effective to hypoxia or reoxygenation-treated cells than that to the cells under normoxia condition. CONCLUSION: These results indicate that SM effectively decreases the abnormal raised amplitude of ICa-L in ventricular myocytes under hypoxia or reoxygenation conditions, preventing Ca^2+ overloaded in the cells. [

关 键 词：心肌 细胞 丹参 钙通道 L-型 钙超载 

分 类 号：R363[医药卫生—病理学]