部分液体通气对急性肺损伤小猪肺组织结构及气体交换的影响  

Effect of partial liquid ventilation with PFC on gas exchange and lung histopathology in piglets with acute lung injury

作　　者：彭雪梅[1] 孙婵[1] 王华东[2] 肖燕青[3] 王仲红[1] 颜亮[2] 王彦平[2] 

机构地区：[1]暨南大学附属第一医院麻醉科,广东广州510630 [2]暨南大学医学院病理生理教研室,广东广州510630 [3]暨南大学附属第一医院检验科,广东广州510630

出　　处：《中国病理生理杂志》2005年第1期129-133,共5页Chinese Journal of Pathophysiology

基　　金：广东省医学科研基金课题(No.A2004348);2003年暨南大学校基金青年项目;广东省自然科学基金资助项目(No.021178)

摘　　要：目的探讨以perfluorocarbon(PFC)为媒介的部分液体通气(PLV)对灌洗后急性肺损伤小猪肺组织病理变化及气体交换的影响。方法采用肺灌洗诱导小猪急性肺损伤(ALI)后,将动物分为两组PLV组和传统机械通气(CV)组。分别在ALI前、ALI及ALI后1、2、3、4h6个时点观察动脉血气指标的变化,实验结束后取8个不同部位的肺组织做病理切片。结果PLV组灌注PFC后PaO2高于CV组、AaDO2显著低于CV组;病理切片比较PLV组病变轻于CV组,两组上部肺叶病变均轻于下部肺叶,具有统计学差异(P<005),CV组前部肺叶病变轻于后部肺叶,具有统计学差异(P<005),PLV组肺组织炎症细胞浸润程度明显轻于CV组肺组织。结论急性肺损伤后,以PFC为媒介的部分液体通气能明显提高肺氧合能力,升高PaO2。AIM: To observe the effect of partial liquid ventilation on gas exchange and histopathological changes of lung in acute lung injury. METHODS: After acute lung injury (ALI) was induced by saline lavage,twenty piglets were assigned randomly to 2 groups: partial liquid ventilation with perfluorocarbon (PFC) group (PLV) and conventional gas ventilation group (CV). The changes of gas exchange were examined before ALI, during ALI and at 1,2,3,4 h after ALI, and histological sections taken from 8 different parts of lung were stained by H & E. RESULTS: The PaO_2 significantly increased and alveolar-aterial oxygen gradient (AaDO_2) markedly decreased in PLV group compare with CV group (P<0.05); the histopathological lesions of lung were less severe in PLV group than CV group, and less severe in upside,foreside of lung than downside, backside of lung in two groups (P<0.05). Moreover, the inflammation of lung was inhibited in PLV group compared with CV group. CONCLUSION: Partial liquid ventilation with PFC can inhibit inflammation in the lung, improve the oxygenation of lung and increase the PaO_2 during acute lung injury. [

关 键 词：部分液体通气 氟碳 急性肺损伤 病理 

分 类 号：R363[医药卫生—病理学]