急性多发脑梗塞大鼠海马皮层MARCKS蛋白的表达与活性的变化  

Overexpression and constitutive activation of MARCKS in brain of rats with multi-cerebral infarction

作　　者：白文[1] 张允岭[2] 韩振蕴[2] 郑宏[2] 黄启福[2] 

机构地区：[1]北京大学人民医院中医科,北京100044 [2]北京中医药大学东方医院神经内科,北京100078

出　　处：《中国病理生理杂志》2005年第1期134-138,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30171196);教育部优秀青年教师基金资助项目

摘　　要：目的研究急性多发脑梗塞大鼠海马皮层MARCKS及p-MARCKS的表达及活性变化,进一步探讨急性脑缺血损害与MARCKS信号转导通路动态变化的关系。方法采用改良Kaneko法建立急性多发脑梗塞动物模型,观察造模后大鼠的神经系统症状体征积分改变及海马组织的病理变化及细胞超微结构的改变;应用免疫组织化学和固定化蛋白印迹法检测MARCKS及其磷酸化在大鼠模型海马及皮层中的表达。结果及结论MARCKS及p-MARCKS在大鼠造模后3d海马及皮层中表达明显高于正常对照组(P<005),这种异常表达与脑缺血损害发生有关。AIM: The purpose of this study was to investigate the expression of myristoylated alanine-rich C kinase substrate (MARCKS) and p-MARCKS during cerebral ischemia. METHODS: The multi-cerebral infarction model was established by method of Kaneko. The stroke symptoms and signs scoring system were used to evaluate the animal model. Microscope and electronic microscope were used to watch the structure of rat brain. The expression of MARCKS and p-MARCKS was measured by Western blotting. The expression pattern of these proteins was further analyzed for their distribution at cellular level by immunohistochemistry staining of the tissues. RESULTS AND CONCLUSION: The expression of MARCKS and p-MARCKS protein in acute ischemic brain were elevated compared with the normal and control group (P<0.05), and the overexpression of these proteins may be correlated with injury of brain tissues. [

关 键 词：多发性脑梗塞 MARCKS 磷酸化 

分 类 号：R363[医药卫生—病理学]