人IFN-β基因脂质体转染胶质瘤细胞对凋亡的诱导作用  

Induction of apoptosis of glioma cell line SHG44 by transfection of human interferon-β gene liposome

作　　者：林伟[1] 章翔[1] 王占祥[1] 费舟[1] 张剑宁[1] 付洛安[1] 刘卫平[1] 郭衍[1] 王西玲[1] 梁景文[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,陕西西安710033

出　　处：《第四军医大学学报》2005年第1期53-56,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金 (30 370 51 2 )

摘　　要：目的 :观察 β 干扰素 (IFN β)基因脂质体pSV2IFN β转染人胶质瘤细胞系SHG4 4及其转染后对SHG4 4细胞的凋亡诱导作用 .方法 :应用MTT比色法检测脂质体pSV2IFN β转染后转染组与对照组SHG4 4细胞增殖的差异 ;细胞免疫荧光染色检测脂质体pSV2IFN β转染后 ,SHG4 4细胞IFN β的表达情况 .应用流式细胞仪Annexin法检测脂质体pSV2IFN β转染后SHG4 4细胞的凋亡情况 ,采用透射电镜观察肿瘤细胞凋亡的形态学改变 .结果 :IFN β基因脂质体pSV2IFN β转染SHG4 4胶质瘤细胞系后 4d和 6d时 ,对肿瘤细胞具有明显的增殖抑制作用 ,抑制率分别为 16 .7%和 32 .7% .细胞免疫荧光法检测表明转染后胶质瘤细胞具有显著的IFN β表达 ,流式细胞仪Annexin法检测表明转染组与对照组的凋亡率分别为 6 6 .8%与 19.8% ,两组相比具有显著差异 (P <0 .0 1) ;透射电镜观察 ,发现有凋亡细胞存在 ,表现为细胞皱缩 ,染色质边集 .结论 :IFN β基因脂质体pSV2IFN β可转染SHG4 4胶质瘤细胞系 ,并对胶质瘤细胞的凋亡具有明显的诱导作用 .AIM: To investigate the transfection of liposome containing pSV2IFN-β and its effects on the induction of apoptosis of human glioma cell line SHG44. METHODS: MTT assay was used to measure the proliferation of SHG44 cells transfected by human interferon-β liposome containing pSV2IFN-β and the IFN-β expression in SHG44 cells was observed by indirect immunofluorescence staining. FCM Annexin was used to detect the apoptotic SHG44 cells after transfection with pSV2IFN-β and the morphological changes of SHG44 cells were detected by electron microscopy. RESULTS: The proliferation of SHG44 was obviously inhibited after transfection of human interferon-β liposome containing pSV2IFN-β and the inhibition rates were 16.7% and 32.7% respectively on d 4 and d 6 after transfection. The results of indirect immunofluorescence staining showed that the IFN-β expressions of SHG44 cells were obvious after transfection and the results of FCM showed that the apoptotic cell rate of the transfection group and control group were 66.8% and 19.8%, respectively. Significant differences were found between the two groups (P<0.01). Morphological changes of apoptosis were also found by electron microscopy. CONCLUSION: Human interferon-β gene liposome containing pSV2IFN-β can transfect human glioma cell line SHG44 and significantly induce the apoptosis of cells line SHG44.

关 键 词：Β-干扰素 脂质体 转染 胶质瘤细胞 凋亡 

分 类 号：R739.41[医药卫生—肿瘤]