奥曲肽和三苯氧胺治疗二甲基苯蒽诱发大鼠乳腺癌的实验研究  

Effect of tamoxifen plus octreotide on DMBA-induced mammary tumors in rats

作　　者：曾希志[1] 姚榛祥[1] 

机构地区：[1]重庆医科大学附属第一医院普外科,400016

出　　处：《中华医学杂志》2005年第1期28-32,共5页National Medical Journal of China

摘　　要：目的　探讨生长抑素类似物奥曲肽(OCT)和三苯氧胺(TAM)的体内抗乳腺癌作用。方法　将接受致癌剂二甲基苯蒽(DMBA, 100mg/kg,一次性皮下注射)后8周的96只Wistar雌鼠,随机分为对照组(单纯诱发组),OCT组( 100μg/kg, 2次/日皮下注射,共14周),TAM组( 1mg/kg,5次/周皮下注射,共14周)和OCT加TAM组,每组24只。观察乳腺肿瘤发生的潜伏期、诱发率,每只大鼠乳腺肿瘤的个数和体积,并利用光镜、电镜进行肿瘤细胞形态学和超微结构研究。结果　(1)乳腺肿瘤平均潜伏期:TAM组(119d±19d)和OCT加TAM组(120d±16d)明显长于对照组(90d±17d)和OCT组(102d±15d) (均P<0 .05); (2)乳腺肿瘤诱发率:OCT加TAM组(23 .8% )、TAM组(45 .5% )、OCT组(52. 4% )均明显低于对照组(70% ) (均P<0 .05 ),而OCT加TAM组明显低于OCT组和TAM组(均P<0 .05); (3)乳腺肿瘤个数:对照组明显高于其余3组(均P<0 .05),OCT加TAM组明显低于OCT组和TAM组(均P<0 .05); (4)注射致癌剂后22周乳腺肿瘤平均体积:对照组(6434mm3 ±1536mm3 )明显高于OCT组(4366mm3 ±1213mm3 )、TAM组(41.38mm3 ±10.91mm3 )和OCT加TAM组(12.85mm3 ±3.29mm3 ) (均P<0 .05),OCT加TAM组明显低于TAM组和OCT组(均P<0 .01);实验18周时TAM组乳腺肿瘤体积(1126mm3 )Objective To further study the antitumor effects of TAM and somatostatin (SST) analog octreotide (OCT) in vivo.Methods Eight weeks following dimethylbenzanthracene (DMBA,a single dose 100 mg/kg by subcutaneous injection) adiministration, 96 Wistar rats were randomly divided into four groups: control group, OCT(100 μg/kg bid for 14 weeks by subc utaneous injection) group, TAM(1 mg/kg 5 times weekly for 14 weeks by subcutaneo us injection) group and OCT+TAM group. The mean latent phases of mammary tumori genesis and incidence of mammary tumor-positive rats were observed. The number and volumes of tumors per animal were measured. The histological structures and ultrastructures of mammary samples were observed by using a light microscopy and a transmission electron microscopy.Results (1) The latent phases of mamary tumorigenesis in the TAM group and th e OCT +TAM group were significantly longer than those in the control or OCT grou p (all P<0.05). (2) The incidence of mammary tumor-positive rats were 70% in the control group, 52.4% in the OCT group, 45.5% in TAM group and 23.8% i n the OCT+TAM group respectively, significantly longer in the three treated grou ps(P<0.05 or P<0.01), and the differences between the OCT+TAM group and the OCT group or TAM group were significant(both P<0.05). (3) The nu mbers of mammary tumors per rat were markedly less in the three treated groups t han in the control group(P<0.05 or P<0.01), and there were significan tly differences between the OCT+TAM group and the OCT group or TAM group(all P<0.05). (4) The mean volumes of mammary tumors per rat were significantly greater in the control group (6434mm3) than in the three treated groups group (P<0.05 or P<0.01), but the tumor volume in the OCT+TAM group (1285 mm3) was less than those in the OCT group (4366 mm3) or TAM group(4138 mm 3) (P<0.01). At 10th week of treatment the mean volume was obviously sma ller in TAM group than in OCT group(P<0.05), but at 14th week of treatmen t the difference was not significant (P>0.05).

关 键 词：TAM OCT 乳腺肿瘤 对照组 乳腺癌 诱发率 大鼠 治疗 生长 发生 

分 类 号：R737.9[医药卫生—肿瘤]