吸烟大鼠脑血管内皮细胞P-选择素、E-选择素表达在脑血栓形成中的作用(英文)  

Effect of cigarette smoking on expression of P-selectin and E-selectin on cerebrovascular endothelial cells in rat's cerebral thrombosis

作　　者：柳忠兰[1] 冯昱[1] 刘淑杰[1] 刘菲[1] 耿建红[1] 

机构地区：[1]中国医科大学附属第一医院,辽宁沈阳110001

出　　处：《中国现代医学杂志》2005年第1期34-37,共4页China Journal of Modern Medicine

基　　金：Supported by grantsfromHealthyMinistryNaturalScienceFoundationofChinaandLiaoningScienceandThchnologyFoundationofChina

摘　　要：目的观察吸烟大鼠脑动脉内皮细胞P-选择素、E-选择素表达,探讨吸烟致脑血栓形成的机制。方法建立吸烟动物模型,将50只大鼠随机分为正常对照组,短期大量吸烟组,长期小量吸烟组,长期大量吸烟组,戒烟组。免疫组织化学法检测大鼠脑动脉内皮细胞P-选择素、E-选择素和TNF-α的表达。结果吸烟大鼠脑血管内皮细胞P-选择素、E-选择素和TNF-α表达较不吸烟鼠明显增加(P<0.05)。长期大量吸烟组P-选择素、E-选择素和TNF-α表达比其他各组明显增多,戒烟组P-选择素、E-选择素和TNF-α表达下降,与其他各吸烟组相比有显著性差异(P<0.05)。结论吸烟致大鼠脑血管内皮细胞P-选择素、E-选择素和TNF-α表达升高是脑血栓形成的重要机制之一。To observe the expression of P-selectin and E-selectin on cerebrovascular endothelial cells of smoking rats in order to investigate pathogenesis of cerebral thrombosis induced by cigarette smoking. Animal model of smoking rats were made, 50 male wistar rats were randomly grouped into normal control, short-term massive, long-term trifle, long-term massive and anti-smoking. Immunohistochemical technique was used to detect the expression of P-selectin, E-selectin and TNF-α in rat's cerebrovascular endothelial cells. Compared with normal control, expression of P-selectin, E-selectin and TNF-α was increased notably in smoking groups (P <0.05). Expression of P-selectin, E-selectin and TNF-αin long-term massive smoking rats was significantly stronger than other groups. Expression of P-selectin, E-selectin and TNF-αwas decreased after anti-smoking and there were sifnificant differences compared with other groups (P <0.05). [Conclusion] Cigarette smoking increases expression of P-selectin, E-selectin and TNF-αin rat's cerebrovascular endothelial cells, which may be one of pathogenesises in cerebral thrombosis.

关 键 词：吸烟 脑血管内皮细胞 P选择素 E-选择素 大鼠 

分 类 号：R743.32[医药卫生—神经病学与精神病学]