穿琥宁对致热大鼠下丘脑组织中PGE_2和cAMP含量的影响  

Effects of chuanhuning injection on contents of PGE_2 and cAMP in hypothalamus in fever rats

作　　者：白霞[1] 马玉东[2] 穆洪[3] 范书铎[1] 

机构地区：[1]中国医科大学生理教研室 [2]沈阳医学院附属中心医院ICU科 [3]南开大学医学部,天津300071

出　　处：《中国临床药理学与治疗学》2005年第1期75-78,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :探讨穿琥宁注射液的解热机制。方法 :用酵母混悬液复制大鼠发热模型 ,观察穿琥宁注射液对大鼠体温的影响及其量效关系 ,并采用放免法测定下丘脑中PGE2 和cAMP含量。结果 :发热模型组在皮下注射酵母混悬液 6h时 ,ΔT及TRI6明显高于对照组 (P <0 .0 1) ,穿琥宁 12 5、2 5 0mg·kg-1组的ΔT及穿琥宁 2 5 0mg·kg-1组的TRI6明显低于发热模型组 (P <0 .0 1) ,且穿琥宁 2 5 0mg·kg-1组的ΔT及TRI6低于 12 5mg·kg-1组 (P <0 .0 1)。发热模型组下丘脑中PGE2 和cAMP的含量明显高于对照组(P <0 .0 1) ,而穿琥宁 12 5、2 5 0mg·kg-1组明显低于发热模型组 (P <0 .0 1) ,且穿琥宁 2 5 0mg·kg-1组低于 12 5mg·kg-1组 (P <0 .0 5 )。ΔT与下丘脑中PGE2和cAMP的含量之间呈明显正相关 (P <0 .0 1)。结论 :穿琥宁注射液可通过抑制下丘脑中PGE2 和cAMP含量的升高而发挥解热作用 ,并存在量效关系。AIM : To investigate the antipyretic mechanisms of the chuanhuning injection in rats. METHODS : The fever model was made by suspension of yeast which was injected hypodermically to the rats. The contents of PGE 2 and cAMP in hypothalamus were measured by radioisotope method. RESULTS : The maximal increment in body temperature (ΔT) and 6 h thermal response index(TRI 6) in the model group were significantly higher than those in the control group ( P < 0.01 ), ΔT in chuanhuning 125 mg·kg -1 group and 250 mg·kg -1 group and TRI 6 in chuanhuning 250 mg·kg -1 group were lower than those in the model group( P BZ < 0.01 ), and ΔT and TRI 6 in chuanhuning 250 mg·kg -1 group were lower than those in chuanhuning 125 mg·kg -1 group ( P BZ < 0.01 ). The contents of PGE 2 and cAMP in hypothalamus in model group were more higher than those in the control group ( P < 0.01 ), the contents of PGE 2 and cAMP in the chuanhuning groups were lower than those in the model group ( P < 0.01 ), and the contents of PGE 2 and cAMP in chuanhuning 250 mg·kg -1 group were lower than those in chuanhuning 125 mg·kg -1 group ( P < 0.05 ). ΔT is positively correlative with the contents of PGE 2 and cAMP in hypothalamus ( P BZ < 0.01 ). CONCLUSION : The antipyretic mechanisms of chuanhuning injection may be related to its decreasing the contents of PGE 2 and cAMP in hypothalamus, and the effects are dosage dependence.

关 键 词：穿琥宁注射液 发热 下丘脑 PGE2 cAMP 

分 类 号：R971.1[医药卫生—药品]