羟丁酸钠对大鼠脑缺血-再灌注后c-fos表达的影响  

Effect of soidium oxybate on expression of c-fos in rasts after focal cerebral ischemia-reperfusion injury

作　　者：李梅[1] 谷淑玲[1] 秦伟[1] 段世明[2] 

机构地区：[1]徐州医学院药理学教研室,江苏徐州221002 [2]徐州医学院江苏省麻醉学重点实验室

出　　处：《徐州医学院学报》2005年第1期25-28,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省麻醉学重点实验室开放课题资助项目(K2092)

摘　　要：目的研究羟丁酸钠(SO)对大鼠脑缺血-再灌注损伤的保护作用,并观察该保护作用对Fos蛋白表达的影响。方法采用左侧大脑中动脉插入栓线(MCAO)方法制作大鼠脑缺血-再灌注模型,观察SO(50、100、200mg·kg-1)对脑缺血大鼠神经功能的影响;用免疫组化方法标记各组大鼠脑组织顶叶皮质、尾壳核中c-fos阳性细胞数量。结果SO可降低大鼠局灶性脑缺血再灌注损伤行为学评分,改善因缺血所致神经行为功能缺失,且随着剂量的增加效应增强,并可以显著减少顶叶皮质、尾壳核Fos阳性细胞的数量(P<0.01)。结论SO对脑缺血损伤有一定的保护作用,且这种作用与其减少脑缺血早期c-fos表达有关。Objective To study the protective effect of sodium oxybate (SO) against focal cerebral ischemia-reperfusion injury in rats and the expression of fos protein and its implication in MCAO rat.?Methods The reversible middle cerebral artery occlusion (MCAO)model was established in rats to investigate the role of SO in ischemia injury. The scores of neurological deficits were determined by Longa EZ method in MCAO rats. The fos protein positive neurons revealed by immunohistochemistry were counted in the PC and Cpu areas.?Results The scores of neurological deficits and the count of fos protein positive neurons were markedly decreased by SO in a dose-dependent manner when the dosage of SO was increased from 50 to100 and 200 mg·kg^-1 (P<0.01).?Conclusion SO can protect MCAO rats against cerebral ischemia-reperfusion injury, the protective effect is related to the expression of c-fos.

关 键 词：大鼠 C-FOS 脑缺血-再灌注 羟丁酸钠 表达 保护作用 顶叶皮质 SO 标记 增加 

分 类 号：R743[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]