过氧化氢酶对SW480细胞内细胞因子和NF-κB激活的影响  

Effects of catalase on expression of cytokines and activation of NF-KB in SW480 cells

作　　者：王群英[1] 王继德[1] 钟世顺[1] 李良仁[1] 赖卓胜[1] 张振书[1] 张亚历[1] 

机构地区：[1]广州南方医科大学南方医院消化疾病研究所,510515

出　　处：《现代消化及介入诊疗》2004年第4期192-194,共3页Modern Interventional Diagnosis and Treatment in Gastroenterology

摘　　要：目的观察过氧化氢酶(catalase,CAT)对脂多糖(Liposaccharide,LPS)诱导肠癌细胞株SW4430细胞内TNF-α、IL-1β、IL-8的表达和NF-κB激活的影响。方法LPS刺激SW480细胞后,采用逆转录-聚合酶链反应(RT-PCR)技术,检测细胞内TNF-α、IL-1β、IL-8 mRNA的表达水平;应用免疫电泳迁移率变动分析(Electrophoretic mobility shift assay,EMSA)法检测细胞内NF-κB的激活。结果CAT 预孵后,TNF-α(正常对照组0.00±0.01,LPS组1.33±0.94,CAT预孵组0.65±0.59,CAT处理组1.31±0.07)、IL-1β(正常对照组0.00±0.05,LPS组0.83±0.05,CAT预孵组0.32±0.06,CAT处理组0.82±0.04)、IL-8(正常对照组0.00±0.01,LPS组1.03±0.20,CAT预孵组0.62±0.25,CAT处理组0.98±0.23)的表达较LPS组和CAT处理组明显降低(P<0.05)。NF-κB的核结合活性也减弱,但较正常对照组仍明显增强。结论CAT能降低SW480细胞对应激的反应,这一作用可能是通过抑制NF-κB的核结合活性,进而降低致炎细胞因子的表达完成的。Objective To investigate the effects of catalase on expression of cytokines and activation of nuclear factor B in intestinal epithelial cells. Methods SW480 cells were stimulated by Liposaccharide (LPS). The expression of TNF-α, IL-1β[, IL-8 in the cells was detected by a semi -quantitative assay, RT-PCR. The activation of NF-κB in cells was evaluated by electrophoretic mobility shift assay (EMSA). Results Comparing with the cells treated by LPS alone, the expression of TNF-α (control 0.00 ± 0.01, LPS 1.33 ± 0.94, CAT pre-treated 0.65 q± 0.59, CAT treated 1.31 ± 0.07), IL-1β (control 0.00 ± 0.05, LPS 0.83 ± 0.05, CAT pre-treated 0.32 ± 0.06, CAT treated 0.82 ± 0.04), IL-8 (control 0.00 ± 0.01, LPS 1.03 ± 0.20, CAT pre-treated 0.62 ± 0.25, CAT treated 0.98 ± 0.23) were significantly decreased (P < 0.05). The activation of NF-κB was also suppressed. Conclusion Catalase could reduce the reaction of stress in intestinal epithelial cells which was probably due to the suppression of the activity of NF-κB and the subsequent inhibiton of the expression of proinflammatory cytokines.

关 键 词：NF—κB LPS SW480 细胞内 正常 对照组 激活 CAT 表达 IL—1β 

分 类 号：R735[医药卫生—肿瘤] R392[医药卫生—临床医学]