骨髓基质细胞与珊瑚人工骨和两种屏障膜生物相容性的实验研究  

The biocompatibility of natural coral and two bioabsorbable barrier membranes with Bone marrow stromal cells

作　　者：唐志英[1] 凌均棨[1] 高燕[1] 邓雨泉[1] 

机构地区：[1]中山大学光华口腔医学院附属口腔医院,医师助教广东510060

出　　处：《中华老年口腔医学杂志》2004年第4期193-196,共4页Chinese Journal of Geriatric Dentistry

摘　　要：目的:探讨珊瑚人工骨(natural coral,NC)作为骨组织工程学载体吸附骨髓基质细胞(bone marrowstromalcells,BMSCs)的可行性,并比较两种可降解性屏障膜PLGA-NC复合膜和PLGA膜的生物相容性及用于牙周引导组织再生术的可行性。方法: 体外分离培养狗的BMSCs,检测其成骨活性;将NC和可降解性屏障膜材料分别吸附原代培养的BMSCs,观察复合物的形态,进行细胞蛋白含量测定。结果: BMSCs在NC的孔隙中生长良好,并有细胞基质的分泌;两种屏障膜与细胞均有较好的生物相容性,但PLGA-NC 复合膜较PLGA膜有更多的微孔和更好的成形性。结论: NC可作为牙周骨组织工程学中的细胞载体;PLGA-NC复合膜比单纯的PLGA膜更适合作为牙周引导组织再生的屏障膜材料。Objective: To study the possibility of natural coral (NC) acting as a carrier of bone tissue-engineering ,compare the physical characters and the biocompatibility with BMSCs of two bioabsorbable barrier membranes:PLGA-NC complex membrane and PLGA membrane, and evaluate the availability of appling them to periodontal guided tissue regeneration(GTR).Methods: BMSCs of primary culture were seeded into porous natural coral particles and two bioabsorbable barrier membranes, PLGA-NC complex membrane and PLGA membrane respectively, the morphological characters of BMSCs combined with NC particles and barrier membranes were observed with phase contrast microscope and scanning electron microscope (SEM).Protein content of cells were detected. Result: BMSCs could enter the pores of natural coral particles and grew well, associating with bone matrix secreting. There was a good biocompatibility between the two bioabsorbable barrier membranes and BMSCs.As to the physical characters, the PLGA-NC complex membrane had more micropores and better plasticity than the PLGA membrane. Conclusions: NC can act as the candidate of bone graft material in bone tissue-engineering of periodontal regeneration. The characters of the PLGA-NC complex membrane was superior to the PLGA membrane as barrier membrane material of GTR.

关 键 词：骨髓基质细胞 可降解性膜 珊瑚人工骨 生物相容性 

分 类 号：R781.4[医药卫生—口腔医学] R783.1[医药卫生—临床医学]