Fas基因转染对舌鳞癌Tca8113细胞生物学特性的影响  

Effect of fas gene transfection on biological characteristics of tongue squamous cell carcinoma cell line Tca8113 cells

作　　者：侯劲松[1] 黄洪章[2] 王建广[2] 潘朝斌[2] 唐海阔[1] 

机构地区：[1]中山大学光华口腔医学院附属口腔医院,主治医师讲师广东510055 [2]中山大学附属第二医院,院长教授广东510120

出　　处：《中华老年口腔医学杂志》2004年第4期202-204,共3页Chinese Journal of Geriatric Dentistry

基　　金：广东省自然科学基金资助项目(A000099013)

摘　　要：目的:研究脂肪酸合成酶(fatty acid synthase,Fas)基因转染对舌鳞癌Tca8113细胞生物学特性的影响,探讨分子机制。方法:脂质体法将含Fas基因的真核表达重组质粒pBK-Fas导入Tca8113细胞,流式细胞术(FCM)检测Fas蛋白表达,MTT法检测细胞化疗敏感性,TUNEL法检测细胞凋亡敏感性,H33342释放法检测外周血单核细胞(PBMC)的癌细胞杀伤活性。结果:Fas转染细胞蛋白表达强度由35.01±5.26提高到55.40±7.31,二者差异有显著性(P<0.01)。相同浓度条件下,5-Fu 对Fas转染细胞杀伤率提高。Fas转染细胞对抗Fas单克隆抗体诱导的细胞凋亡敏感性增强,凋亡指数由16.88%±1.46%提高到27.12%±2.35%。与未转染细胞相比,两项指标差异均有显著性(P< 0.01)。PBMC对转染细胞的杀伤活性为51.22%±4.61%,对未转染细胞为23.92%±2.38%,差异也有显著性(P< 0.01)。结论:Fas基因转染提高化疗药物和机体免疫细胞对舌鳞癌细胞的杀伤活性。Objective:To investigate effect of fatty acid synthase(fas)gene transfection on biological characteristics of tongue squamous cell carcinoma cell line Tca8113 and its molecular mechanism. Methods: Plasmid including fas gene was transfected into Tca8113 cells by lipofectamine kit. Fas protein expression in tumor cells was detected by flow cytometry(FCM). Cell's response to 5-Fu was assayed by MTT technique. Apoptosis in tumor cells was examined by TUNEL. And PBMC killing activity against carcinoma cells was evaluated by H33342 release procedure. Results:fas gene transfection regulated fas protein expression intensity from 35.01±5.26 in untrasfected cells up to 55.40±7.31 in transfected cells. Under the same concentration of 5-Fu and anti-fas antibody, cell death and apoptosis index (AI) increased in fas transfection cells. tatistically, The difference of cell killing rate and AI between untransfected(16.88%±1.46% )and transfected cells(27.12%±2.35%)were significant(P<0.01). PBMC killing activity on untransfected and transfected Tca8113 cells were 23.92%±2.38% and 51.22%±4.61% respectively and also had significant difference(P<0.01). Conclusions: Fas gene transfection enhances killing ability of chemotherapy drug and facilitates immunological cell against oral squamous cell carcinoma cells.

关 键 词：口癌 舌癌 鳞状细胞 脂肪酸合成酶 基因转染 生物学特性 细胞凋亡 

分 类 号：R739.86[医药卫生—肿瘤]