妊马雌酮对大鼠体外培养成骨细胞作用的研究  

Effects of premarin on rat osteoblasts in vitro

作　　者：韩庆国[1] 

机构地区：[1]深圳大学生命科学院,518060

出　　处：《中国骨质疏松杂志》2004年第4期477-479,482,共4页Chinese Journal of Osteoporosis

摘　　要：目的 探讨妊马雌酮对大鼠体外培养成骨细胞的作用效果及对成骨细胞增殖的作用机理。方法采用新生大鼠颅骨进行分离成骨细胞,并在含10％的胎牛血清DMEM中进行体外培养,分别加入高、低剂量妊马雌酮并与阴性组进行对照,用MTT法进行成骨细胞增殖和活性检测,用Westem免疫印迹检测蛋白激酶C、抗凋亡蛋白Bag-1表达。结果 在培养24 h后,妊马雌酮对培养成骨细胞增殖有明显的促进作用,培养到48 h促进作用更为明显,表现出一定的时间相关性。妊马雌酮能明显提高体外成骨细胞PKC的表达量和抗凋亡蛋白Bag-1的表达量。结论 妊马雌酮可促进成骨细胞增殖并明显提高PKC和抗凋亡蛋白Bag-1的表达量是其防治骨质疏松的机理之一。Objective To study the effect and mechanism of premarin on rat osteoblasts in vitro. Methods The osteoblasts were separated from the skull of new-born rats, and were cultured in 10% bovine serum DMEM. High and low dosage premarin was separately added and compared with blank group. In the end, osteoblasts were tested through MTT method. Expression of PKC and Bag-1 were assayed by Western-blot. Results Premarin promoted the multiplication of osteoblats cultured for 24 hours while the effect was even strengthened after 48 hours. Expression quantity of PKC and anti-apoptosis protein Bag-1 was increased remarkably. Conclusions Premarin prevents and treats osteoporosis by promoting multiplication of osteoblasts and enhancing the expression of PKC and anti-apoptosis protein Bag-1.

关 键 词：成骨细胞 体外培养 增殖 雌酮 BAG-1 大鼠 抗凋亡蛋白 PKC 阴性 MTT法 

分 类 号：R737[医药卫生—肿瘤] R446.6[医药卫生—临床医学]