脐血血浆化疗增敏作用的实验研究  

Experimental study on the sensitizing effect of the cord blood plasma to chemotherapy

作　　者：姚一芸[1] 程毅敏[1] 朱琦[1] 叶为德[1] 唐勇[1] 胡钧培[1] 

机构地区：[1]上海第二医科大学附属第九人民医院血液科,上海200011

出　　处：《诊断学理论与实践》2004年第6期435-437,共3页Journal of Diagnostics Concepts & Practice

摘　　要：目的:研究脐血血浆(CBP)对急性髓细胞性白血病(AML)细胞的体外化疗增敏作用。方法:以AML细胞株HL鄄60细胞为实验对象,培养体系中分别加入CBP或人重组粒鄄巨噬细胞集落刺激因子(rhGM鄄CSF)作为实验组或阳性对照组,不加CBP和rhGM鄄CSF的HL鄄60细胞作为阴性对照组。培养48h后进行细胞周期动力学检测;各组细胞再加入阿糖胞苷(Ara鄄C)培养,24h后进行细胞增殖测定。结果:经过48h孵育,CBP组S期细胞比例明显增高,再与Ara鄄C作用,细胞的生长较阴性对照组明显受抑。实验组和阳性对照组之间无明显差异。结论:CBP与rhGM鄄CSF相似,可通过促进HL鄄60细胞进入S期增强对Ara鄄C的敏感性。Objective To study the effect of the cord blood plasma (CBP) in sensitizing the response of the acute myelocytic leukemia (AML) cell line HL-60 to chemotherapy in vitro. Methods The AML cell line HL-60 was cultured in different media: containing CBP as the experimental group or rhGM-CSF as the positive contnol or without either CBP or rhGM-CSF as the negative control. After 48 h culture, the cell cycle kinetics were detected. And then Ara-C was added in the media of each gronp. After 24 h culture, cell proliferation assessment was performed. Results The percentage of S phase cells in the experimental group elevated significantly. After the treatmemt of cytosine arabinoside (Ara-C), the cell growth in the experimental group was suppressed significantly in comparison with that in the negative controls. There was no statistical difference between the experimental group and the positive contnols. Conclusions CBP might enhance the sensitivity of HL-60 cells to the chemotherapy which is similar to rhGM-CSF, throgh promoting the HL-60 cells into S phase.

关 键 词：脐血血浆 化疗 阿糖胞苷 重组人粒-巨噬细胞集落刺激因子 增敏作用 实验 急性髓细胞性白血病 

分 类 号：R733.71[医药卫生—肿瘤] R73-361[医药卫生—临床医学]