九种蟋蟀mtDNA-16S rRNA基因序列及其系统进化(直翅目:蟋蟀科)  

Phylogenetic Relationships of Nine Crickets Based on Partial Sequences of 16S rRNA Gene (Orthoptera:Gryllidae)

作　　者：蒋国芳[1] 陈爱辉[1] 冯金叶[1] 

机构地区：[1]南京师范大学生命科学学院江苏省资源生物技术重点实验室,江苏南京210097

出　　处：《Entomotaxonomia》2004年第4期249-254,共6页昆虫分类学报（英文）

基　　金：国家自然科学基金资助项目(30160015)

摘　　要：蟋蟀科5属9个种的线粒体16SrRNA基因部分序列被测定或从GenBank获得,比较其同源性,计算核苷酸使用频率,并构建NJ和MP分子系统树。在获得的449bp的序列中A、T、C和G碱基含量分别为31.8%、36.9%、9.9%和21.4%,A+T平均含量为68.7%。研究结果表明:所研究的5属9种蟋蟀聚成3个聚类簇,斗蟋属先与灶蟋属汇合,再与棺头蟋属构成聚类簇I;油葫芦属黑脸油葫芦和北京油葫芦与蟋蟀属的家蟋相聚构成聚类簇II;蟋蟀属的田蟋单独构成聚类簇III。The partial sequences of mitochondrial 16S rRNA gene (449bp) were sequenced for 9 species of crickets belonging to 5 genera of Gryllidae. The homologous sequences were compared, the used frequency of nucleotide was calculated and the molecular trees reconstructed by Neighbor-Joining method and maximum parsimony method using Dianemobius taprobanensis as outgroup. In the obtained sequences of crickets, the content of A, T, C and G was 31.8%, 36.9%, 9.9% and 21.4% respectively, and A% + T% was 68.7%. The reseach results suggested that 9 individuals from the 5 genera clustered in 3 clusters, among which genus Velarifictorus and Gryllodes firstly clustered and then together with genus Loxoblemmus to form cluster I; Cluster II was made of three species, Teleogryllus emma, T. mitratus and Gryllus domesticus; G. campestris single as cluster III.

关 键 词：蟋蟀科 直翅目 系统进化 分子系统树 RRNA基因 序列 碱基 葫芦 同源性 含量 

分 类 号：Q963[生物学—昆虫学]