大鼠根尖周炎中降钙素基因相关肽阳性神经纤维的变化  

Change of calcitonin gene-related peptide immunoreactive nerve fibers in periapical periodontitis of rats

作　　者：曹志中[1] 马凤云[2] 刘宏[1] 刘军[1] 叶晖[1] 

机构地区：[1]第二军医大学长海医院 [2]第二军医大学细胞生物教研室,上海200433

出　　处：《牙体牙髓牙周病学杂志》2004年第12期674-676,共3页Chinese Journal of Conservative Dentistry

基　　金：上海市自然科学基金资助项目(02ZB14001)

摘　　要：目的观察降钙素基因相关肽阳性(CGRP-IR)神经纤维在大鼠根尖周炎中的变化,为进一步研究CGRP在根尖周炎中的作用提供形态学依据。方法在大鼠磨牙制备近髓窝洞,封入新鲜龋坏组织,建立大鼠根尖周炎模型。免疫组织化学技术观察正常根尖周膜和炎症14、21d根尖周组织CGRP-IR神经纤维的变化。结果根尖周炎时,大鼠磨牙根尖周膜CGRP-IR神经纤维增多、增粗、染色加重,并围绕在坏死组织周围。结论根尖周炎时,根尖周膜内CGRP增多,并位于炎症的前沿区,CGRP可能参与了根尖周组织炎症及修复过程。AIM:To investigate the change of calcitonin gene-related peptide immunoreactive(CGRP-IR)nerve fibers in rat periapical periodentium during inflammation,providing morphological data of CGRP in periapical periodontitis.METHODS:Deep cavities were prepared on rats′ molars,and fresh carious dentin was enclosed in it to establish animal periapical periodontitis model.Immunohistochemical technology was used to observe the distribution of CGRP-IR nerve fibers in rats′ normal periodontium and inflammatory periodontium at 14,21 d respectively.~RESULTS: In the periapical periodentium,the CGRP-IR nerve fibers became denser,more heavily stained and surrounding the necrotic zone.CONCLUSION:During periapical periodontitis,the amount of CGRP increased in periapical periodentium,and located in the frontline of inflammation.The results suggested that CGRP may play a role in the inflammation and repairation of periapical periodentium. [

关 键 词：根尖周炎 降钙素基因相关肽 

分 类 号：R780.2[医药卫生—口腔医学]