兔脑缺血灶内胎兔脑干中缝核细胞的移植  

TRANSPLANTATION OF RABBIT FOETAL NEURONS OF RAPHE NUCLEI IN FOCAL BRAIN ISCHEMIA OF ADULT RABBIT

作　　者：张天锡[1] 姜智南 赵卫国[1] 杨践[3] 

机构地区：[1]上海第二医科大学瑞金医院神经外科 [2]宁波市一医院 [3]上海第二医科大学瑞金医院病理科

出　　处：《上海医学》1993年第11期644-647,共4页Shanghai Medical Journal

基　　金：国家自然科学基金

摘　　要：采用改良的 O'Brien 法建立兔大脑中动脉阻断型(MCAO)局灶脑缺血模型。取胎龄18～25天的胎兔脑干中缝核血清素(5-HT)能性神经元,以细胞悬浮液方式应用脑立体定位技术移植到兔脑缺血灶内。分别手移植后1、2、3个月时处死动物取其脑标本进行5-HT 的免疫组化研究。发现兔MCAO 局灶脑缺血模型稳定可靠,所有动物均存在大小不等、程度不同的脑缺血坏死灶。移植的5-HT 阳性细胞能够在脑缺血灶内存活、生长并成熟,同时发出细胞突起与宿主脑细胞形成联系;植入神经元由最初的集聚成簇逐渐向周围脑实质内迁移并与宿主脑整合起来。随着移植后存活时间的延长,植入神经元更趋成熟,脑缺血灶趋于修复吸收。Focal rabbit brain ischemia model(MCAO)was established by electrical cauteriza- tion of MCA via a transorbital approach.Pieces of raphe nuclei-containing serotonergic neurons were dissected from 18-20 days(E18 to E20)rabbit embryos,mechanically dis- sociated,and 20 μl of cell suspension was injected slowly under stereotaxic control into the focus of brain ischemia of adult rabbit.One,two and three months after trans- plantation respectively,the brain of the grafted rabbits were processed for immunohis- tochcmical detection of 5-HT with specific 5-HT antibodies.5-HT neurons in the focus of brain ischemia underwent survival,growth,maturity and exhibited the morphology of an adult neuron with well-developed process(axons and dendrites)extending far from the injection site.This results indicated that the grafted neurons have been integ- rated into the host's neuronal circuity.

关 键 词：脑细胞移植 脑缺血 兔 血清素能 

分 类 号：R743.310.5[医药卫生—神经病学与精神病学]