大鼠坐骨神经移植段对受损的视网膜节细胞影响的形态学研究—存活时间因素的影响  

MORPHOLOGICAL STUDY ON THE EFFECT OF IMPLANTED SCIATIC NERVE SEGMENT ON THE INJURED RETINAL GANGLION CELLS IN THE RAT——EFFECT OF SURVIVAL TIME

作　　者：雷季良[1] 王淑玲[1] 于恩华[1] 

机构地区：[1]北京医科大学解剖学教研室,北京100083

出　　处：《神经解剖学杂志》1993年第2期217-220,共4页Chinese Journal of Neuroanatomy

摘　　要：大鼠15只,先将动物视神经切断,再移植坐骨神经段(PN、长约2mm/每段)到眼玻璃体内,动物分别存活2、4、8周后,取视网膜,沿视轴纵切,Nissl染色,显微镜观察,并用计算机图像分析仪(IBAS-2000)测量部分视网膜节细胞胞体截面积。结果证明,在PN的诱导下,存活2、4、8周时的视网膜节细胞层均出现巨大视神经细胞,胞体呈椭圆形,其胞体平均截面积分别为75.3±16.0μm^2、93.7±33.8μm^2和81.2±19.6μm^2;其中特巨细胞构成比分别为7.6％、34.6％和17.5％;二者均以存活4周时为最大,三组呈现一个由小到大再减小的变化趋势。经统计学检验,三组之间均有显著性差异(P<0.01)。15 rats of male were used in this experiment. At first optic nerve were cut. then a segment of sciatic nerve (PN, 2mm in length/one segment) were implanted into the vitreous body of the eye. Retina were sectioned after animals have survived for 2、4、8 weeks. The sections were stained by Nissl method. The RGCs were observed by a light microscope, and cell areas were measured by the Image Analysis System (IBAS-2000). The experimental results suggest that some giant oval RGCs were found after PN were implanted into the vitreous body and survived for 2、4、8 weeks. The average areas of the giant RGCs in different stages were 75.3+16.0μm^2、93.7±33.8μm^2 and 81.2+19.6μm^2 respectively (P<0.01, t test), and the constituent ratios (100μm^2↑) were 7.6%、34.6% and 17.5% (P<0.01. u test). The numbers including both the average areas and the constituent ratios of those survived 4 weeks were the largest, which suggested a tendency of changing from small to large and then to small.

关 键 词：视网膜节细胞 周围神经 神经再生 

分 类 号：R361.3[医药卫生—病理学]